Restriction Enzymes
Learn about the history of restriction enzymes, usage considerations, troubleshooting and how restriction enzymes are used in non-cloning applications.

Learn about the basics

Overview of restriction enzyme history, naming system and classification are discussed in this section.

Key considerations for reaction set-up

Successful restriction enzyme reactions to prepare DNA for ligation rely on enzyme quality and behavior, star activity, methylation status and reaction set-up.

Troubleshooting guide

This restriction enzyme troubleshooting guide organizes solutions to common issues encountered when performing digestions with restriction endonucleases.

Genome mapping and analysis

Learn about restriction endonuclease applications in genome mapping and analysis such as RFLP, AFLP, COBRA, and qAMP.