When TOPO cloning technology was launched, it sparked a revolution. Over the past 18 years, TOPO technology has become the most widely used cloning method in the world.

TOPO cloning technology is:

  • Efficient—up to 95% of clones contain desired insert
  • Fast—5-minute, room temperature reaction
  • Easy—simple 3-step procedure
  • Proven—over 20,000 citations
  • Flexible—available with or without competent cells, in multiple reaction sizes

No matter what your downstream application is, TOPO cloning technology helps you get the right clone sooner, freeing up your time to answer more important questions.

TOPO cloning technology brochure

See how Invitrogen™ TOPO™ PCR Cloning technology works

Learn how simple it is to get 95% recovery of your clone in just 5 minutes.

Choose the right TOPO® cloning solution for you

TOPO® blunt-end cloning

NEW: 25% extra reactions

For the direct insertion of blunt-end PCR products amplified with proofreading thermostable polymerases into a plasmid vector.

NEW: 25% extra reactions

For the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning.

For easy, efficient cloning of long (3–10 kb) PCR products using the pCR®-XL-TOPO® vector.

TOPO® Kits without competent cells

NEW: 25% extra reactions

Gateway® Recombinant Cloning with TOPO® Technology

Expression cloning with TOPO® Technology

Meet the Inventor

Find out how the revolutionary TOPO® cloning was developed with Dr. John Chestnut, research fellow in Synthetic Biology RND.

Dr. Jon Chesnut
TOPO® Cloning Technology