TOPO cloining kits

Whether you’re PCR cloning with Taq DNA polymerase or a proofreading enzyme, there is a TOPO cloning kit available to take you quickly and efficiently into your downstream application.

Select the correct TOPO Cloning Kit by application

To order, review available kits by application, cloning strategy (TA, blunt, directional), advantages, and catalog numbers.

General subcloning

Product TOPO cloning Advantages One Shot Competent cells Order now
TOPO TA Cloning Kit TA
  • Get your clone with 95% efficiency 
  • Selection of competent cells for high speed, electroporation, or routine cloning
TOPO TA Cloning Kit for Subcloning without Competent Cells 450641
Mach1-T1R K451020
TOP10 K450001
TOP10F' K455001
TOP10 Electrocomp K456001
MAX Efficiency DH5α-T1 Phage-Resistant K452001
Zero Blunt TOPO PCR Cloning Kit Blunt
  • Unique technology minimizes background 
  • Get your clone with 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convenient 
  • Selection of competent cells for high speed, electroporation, or routine cloning
Zero Blunt TOPO PCR Cloning Kit without Competent Cells 450245
Mach1-T1R K283020
TOP10 K280020
TOP10 Electrocomp K286020
MAX Efficiency DH5α-T1 Phage-Resistant K282020

Long PCR fragments

Product TOPO cloning Advantages One Shot Competent cells Order now
NEW TOPO  XL-2 Complete PCR Cloning Kit Blunt
  • Complete kit offering high cloning efficiencies for single PCR fragments up to 13kb in length 
  • Includes Platinum SuperFi Green PCR MasterMix, for exceptional fidelity and specificity
OmniMAX 2 T1R Chemically Competent cells K805020

In vitro transcription

Product TOPO cloning Advantages One Shot Competent cells Order now
TOPO TA Cloning Kit Dual Promoter TA
  • Get your clone with 95% efficiency
  • Dual T7 and SP6 priming sites for in vitro transcription
Mach1-T1R K461020
TOP10F' K460001
TOP10 Electrocomp K466001
MAX Efficiency DH5α -T1 Phage-Resistant K462001

Sequencing

Product TOPO cloning Advantages One Shot Competent cells Order now
TOPO TA Cloning Kit for Sequencing  (pCR 4 TOPO Vector) TA
  • Get your clone with 95% efficiency   
  • Sequence more insert, less vector
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convenient
TOPO TA Cloning Kit for Sequencing without Competent Cells 450030
Mach1-T1R K453020
TOP10 K457501
TOP10 Electrocomp K458001
MAX Efficiency DH5α -T1 Phage-Resistant K289520
Zero Blunt TOPO PCR Cloning Kit for Sequencing (pCR 4 Blunt TOPO Vector) Blunt
  • Unique technology minimizes background 
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
Mach1-T1R K283520
TOP10 K287520
MAX Efficiency DH5α-T1 Phage-Resistant K289520
TOP10 Electrocomp K288020

Expression in E.coli

Product TOPO cloning Advantages One Shot Competent cells Order now
Champion pET100 Series Directional TOPO Expression Kits (pET Directional TOPO Vector) Directional
  • Up to 90% of clones in the correct orientation 
  • High-level protein expression using E. coli 
  • Convenient purification and detection tags
BL21 Star(DE3) K10001
K10101
K10201
K15101

Expression in mammalian cells

Product TOPO cloning Advantages One Shot Competent cells Order now
pcDNA 3.3 TOPO TA Cloning Kit TA
  • 3–5 higher expression compared to to conventional CMV vectors 
  • Ideal for expression of native protein
TOP10 K830001
pcDNA 3.2/V5/GW/D-TOPO Cloning Kit Directional
  • Greater than 90% of clones in correct orientation
  • CMV promoter delivers high-level expression in mammalian cells 
  • C-terminal V5 and 6xHis tags for convenient detection and purification 
  • Neomycin selection
TOP10 K244020
ViraPower HiPerform Lentiviral FastTiter TOPO Expression Kits (pLenti 7.3 Vector) TA
  • Greater than 4X higher expression compared to the original system 
  • Efficient TOPO cloning
Stbl3 K495000

Entry into Gateway Entry Vectors

Product TOPO cloning Advantages One Shot Competent cells Order now
pCR8/GW/TOPO TA Cloning Kit TA
  • Includes plasmid miniprep kit 
  • Fast growing cells for fast miniprep
Mach1-T1R K532000
TA
  • Fast growing cells for fast miniprep
Mach1-T1R K252020
TOP10 K250020
pENTR/TEV/DTOPO Cloning Kit Directional
  • Fast growing cells for fast miniprep
Mach1-T1R K252520
pENTR/D-TOPO Cloning Kit Directional TOP10 K240020
pENTR/SD/DTOPO Cloning Kit Directional TOP10 K242020