TOPO® TA Cloning with Mach1™ Competent Cells
TOPO® cloning kits with Mach1™-T1R competent cells make cloning fast and simple. Mach1™ cells deliver robust growth for miniprep analysis in just four hours, saving up to 2 days compared to restriction enzyme and ligation cloning.
We offer a suite of TOPO® cloning kits with Mach1™-T1R competent cells for basic cloning of Taq-amplified fragments, blunt-ended fragments, sequencing, long-fragment PCR cloning, and entry into the Gateway® system. Simply add your PCR product (blunt-ended fragment or amplified using Taq polymerase) to the appropriate vector, incubate for 5 minutes and transform into our fast growing Mach1™ competent cells.
|Invitrogen's Mach1™-T1R E. coli achieve sufficient growth for miniprep analysis from an overnight colony in just four hours (Figure 1) compared to conventional strains which require an overnight incubation.
Mach1™ competent cells are also available with Zero Blunt® and TOPO® XL vectors: