PCR Clean-Up From Complex Mixtures of DNA
PCR clean-up is a routine but time-consuming laboratory procedure. Now you can use an easier, faster, safer method and get superior results. Purify DNA using a simple and rapid PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.
Isolated DNA is ready for sequencing, PCR, transcription, mapping, cloning, and labeling. We offer a wide range of Invitrogen™ PCR clean-up kits, plus the support you may need to get high yields of pure DNA (Figure 1).
|Figure 1. ChargeSwitch®-Pro kits simplify PCR clean-up. Sequencing results from amplicons purified using the ChargeSwitch®-Pro PCR Clean-Up Kit. The ChargeSwitch®-Pro PCR Clean-Up Kit and QIAquick® PCR Purification Kit were used according to the manufacturers’ directions. A purified 2 kb sample (100 ng) from each purification was used in BigDye® Terminator sequencing reactions with the T7 primer. (A) Electropherogram of a sample purified with the ChargeSwitch®-Pro kit. (B) Phred 20 scores averaged from triplicate samples.|
Which PCR product clean-up kit is right for you?
|Quick and Efficient removal of by-products||Ideal for concentrating low yield PCR products||Simple, reliable, rapid method, 96-well format||Rapid, one tube, 5 minute protocol for high recovery of DNA||Complete kit with no harsh chemicals for fast, hassle-free automatable setup|
|Order now||Order now||Order now||Order now||Order now|
|PureLink® PCR Purification Kit||PureLink® PCR Micro Purification Kit||PureLink® 96 PCR Purification Kit||ChargeSwitch® PCR Clean-Up Kit||ChargeSwitch®-Pro PCR Clean-Up Kit|
|Format||Spin/vacuum column||Spin/vacuum column||96-well plate (vacuum/centrifuge)||Magnetic beads||Spin/vacuum column|
|Protocol time||15 min||15 min||15 min||5 min||5 min|
|Sample volume||50–100 µL||50–100 µL||50–100 µL||25–50 µL|
|Yield plasmid DNA||Up to 40 µg||Up to 20 µg||Up to 20 µg||Up to 30 µg||Up to 15 µg|
|DNA fragment size||100 bp–15 kb||100 bp–15 kb||100 bp–15 kb||90 bp–40 kb||125 bp–12 kb|
|Binding capacity||Up to 40 µg||Up to 40 µg||Up to 40 µg||~25 µg per mg beads||~25 µg per mg beads|
|Downstream Application||Sequencing, Sequencing (Next Generation), Nucleic Acid Labeling, PCR, Cloning||Sequencing, Nucleic Acid Labeling, PCR, Cloning||Sequencing, Cloning||Sequencing, Sequencing (Next Generation), Microarray Analysis, PCR, Cloning||Sequencing, Sequencing (Next Generation), Microarray Analysis, PCR, Cloning|
|High Throughput Compatibility||Yes||No||Yes||Yes||No|
|Pack size||50 preps 250 preps||50 preps 250 preps||4 plates||100 preps 960 preps||50 preps 250 preps|
PureLink® Combo Kit
If you do PCR clean-up and gel extractions, consider our PureLink® combo kit that allows you to do either process with a single kit.
For Research Use Only. Not for use in diagnostic procedures.