The 15 minute size separation and recovery system for library construction
- Size separate free adapters from ligated product 10X faster in 3 easy steps
- Optimized for tight, consistent recovery of DNA fragments <1Kb in size
- Purified DNA is compatible with library construction protocols of major generation 2 sequencing platforms
- Use recovered DNA directly in cloning reactions
Size select DNA in 3 easy steps
With E-Gel® SizeSelect™ gels, you can separate and recover DNA for library construction in 3 easy steps,
E-Gel® SizeSelect™ Gels are double-comb, pre-cast agarose gels with a twist. Load your sample into the top row and electrophorese until your band or desired size range enters the bottom row. Then, easily remove the size-selected DNA with a pipette. That’s it. No additional steps are required.
Reverse-run functionality on the E-Gel® iBase™ Power System lets you capture bands of interest even if you miss the bands when they pass through the collection wells. Watch live migration of DNA with the E-Gel® Safe Imager™ Real Time Transilluminator.
Have DNA ready for library construction 10x faster
The E-Gel® SizeSelect™ gels allow users to separate and recover DNA for short read fragment library construction in just 15 minutes. That's 10 times faster than using conventional gels, and purification kits. E-Gel® SizeSelect™ gels are offered in a 2% agarose concentration, and validated for the recovery of fragments in the 150-200bp size range. Once the DNA is recovered, no additional purification step is required before proceeding to the next step in library construction. E-Gel® SizeSelect™ system has been used successfully in these generation 2 library construction protocols:
- ABI's SOLiD Fragment Library Construction
- ABI's SOLiD Mate Pair Library Construction
- Illumina's Genome Analyzer Fragment Library Construction
- Illumina's Genome Analyzer Paired-end Library Construction
The Fastest and Most Accurate
The E-Gel® SizeSelect™ system is the fastest, most accurate method for the separation and recovery of DNA fragments for library construction using a major generation 2 sequencing platform. The E-Gel® SizeSelect™ system,
- Dramatically reduces the time required to construct libraries
- Provides tight, consistent size recovery of fragments <1Kb in size
The smartest way to gel-purify DNA for cloning reactions
E-Gel® SizeSelect™ gels are the smartest way to gel-purify DNA fragments <1Kb in size for downstream cloning reactions. Purified DNA can be used directly in any cloning reaction without the need for additional purification kits. The proprietary stain incorporated in the E-Gel® SizeSelect™ gels is five times more sensitive than ethidium bromide, allowing maximal retrieval of DNA bands. Avoid the personal hazards of UV exposure by using the Safe Imager™ Real-Time Transilluminator for visualization migrating DNA bands. The blue light produced by the Safe Imager™ Real-Time Transilluminator, ensures integrity of the sample DNA which in turn maximizes cloning efficiency.
||Figure 3. Cloning efficiency with E-Gel® SizeSelect™ Gels and the Safe Imager® blue light transilluminator. PCR-generated inserts of 3 different molecular weights were cloned into pCR® 2.1 TOPO® vector using manufacturer’s protocol. Prior to cloning, the fragments were purified from either E-Gel® incorporated with ethidium bromide, or E-Gel® SizeSelect™ gels. Fragments were retrieved from E-Gels with ethidium bromide by excision using a razor blade followed by column purification. Reactions were performed in triplicates and data are presented as average values. Standard deviations are represented as error bars.