We offer a range of UltraPure agarose and reagents to meet your nucleic acid analysis and purification needs. UltraPure agarose and reagents are made from the highest purity biochemicals for maximum reliability and superior performance.

UltraPure Agarose

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Enjoy the benefits of a quality product in easy-to-use packaging. Our reagent pouches feature a spout that make pouring and dispensing the powder easier, reducing the likelihood of spills and contamination.

UltraPure Agarose is standard melting-point agarose designed for routine separation analysis of DNA and RNA fragments in the 500–23,000 bp range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. Agarose 1000 is easier to handle because it has a stronger gel structure: gel strength >1,400 g/cm2. For low melting/gelling temperature agarose, UltraPure Low Melting Point Agarose is recommended for rapid DNA gel extraction protocols. Listed below are some reasons and tips to use LMP agarose.

Reasons to use low melting point (LMP) agarose

Low melting point (LMP) agarose is ideal for resolving DNA fragments from 10 bp to 1,000 bp. The major advantage of LMP agarose is the low melting temperature of 65°C or less. At these temperatures, it is possible to remelt the agarose of a gel without melting the double helix of DNA molecules in the agarose. Therefore, slices of LMP agarose containing DNA can be melted and the nucleic acids manipulated directly in the remelted agarose.

Applications and references for LMP agarose:

  1. In-gel ligation and transformation: Struhl K (1985) A rapid method for creating recombinant DNA molecules. BioTechniques 3:452–453.
  2. In-gel restriction digestion: Parker RC, Seed B (1980) Two-dimensional agarose gel electrophoresis “SeaPlaque” agarose dimension. Methods Enzymol 65(1):358–363.
  3. In-gel PCR: Bjourson AJ, Cooper JE (1992) Band-stab PCR: a simple technique for the purification of individual PCR products. Nucleic Acids Res 20(17):4675.
  4. Cycle sequencing: Gorman KB, Steinberg RA (1989) Simplified method for selective amplification and direct sequencing of cDNAs. Biotechniques 7(4):326–328, 331.
  5. Blunt-end ligation: Hemsley A, Arnheim N, Toney MD, et al. (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res 17(16):6545–6551.
  6. DNA recovery with β-Agarase: Crouse J and Amorese D (1987) Ethanol precipitation: ammonium acetate as an alternative to sodium acetate. GIBCO-BRL Focus 9:3–5.
  7. DNA recovery with phenol/chloroform: Benson SA (1984) A rapid procedure for isolation of DNA fragments from agarose gels. BioTechniques 2: 66–67.

Tips for using LMP agarose:

  • Use 1x TAE buffer instead of 1x TBE buffer
  • Stain the gel after electrophoresis with SYBR Safe DNA gel stain. Ethidium bromide staining can result in DNA degradation.
  • Visualize DNA with UV light source ≥300 nm and minimize exposure to less than 1 minute.
  • Melt the gel slice at 65°C. Do not exceed 70°C, which may result in DNA denaturation.

UltraPure Reagents

To make it easier to find your reagent of choice, we have grouped our UltraPure reagents by application and common reagents.


Agarose and polyacrylamide gel electrophoresis

Product Quantity Cat. No.
 UltraPure 10X TBE Buffer  1 L  15581044
 UltraPure 10 mg/mL Ethidium Bromide  10 mL  15585011
 UltraPure DNase/RNase-Free Distilled Water  10 x 500 mL  10977023


Southern blot analysis

Product Quantity Cat. No.
 UltraPure Salmon Sperm DNA Solution  5 x 1 mL  15632011
 UltraPure 20X SSC  4 L  15557036
 UltraPure Tris HCl  500 g  15506017
 UltraPure 5M NaCl  10 L  24740011
 50X Denhardt’s Solution  100 mL  750018


Enzymes

Product Quantity Cat. No.
 RNase A  10 mL  12091021
 RNase A  25 mL  12091039
 Proteinase K  100 mg  25530015
 Proteinase K  1 g  25530031
 Proteinase K  5 mL  25530049


Northern blot analysis

Product Quantity Cat. No.
 UltraPure DEPC-Treated Water  1 L  750023
 UltraPure 0.5 M EDTA, pH 8.0  500 g  15576028
 UltraPure 5 M NaCl  10 L  24740011
 UltraPure Tris HCl  500 g  15506017
 UltraPure 20X SSC  4 L  15557036
 UltraPure 20X SSPE  4 L  15591035
 UltraPure 10% SDS Solution  4 x 100 mL  15553027
 UltraPure Formamide  500 g  15515026
 UltraPure DNase/RNase-Free Distilled Water  10 x 500 mL  10977023
 UltraPure Herring Sperm DNA Solution  5 x 1 mL  15634017
 50X Denhardt’s Solution  100 mL  750018


Phenol products

Product Quantity Cat. No.
 UltraPure Phenol  500 g  15509037
 UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)  100 mL  15593031
 UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)  400 mL  15593049
 UltraPure Phenol:Water (3.75:1, v/v)  400 mL  15594047


Common reagents

Product Quantity Cat. No.
 UltraPure 0.5 M EDTA, pH 8.0  500 g  15576028
 UltraPure 10% SDS Solution  1 L  24730020
 UltraPure 10X TAE Buffer  4 L  15558026
 UltraPure 10X TBE Buffer  1 L  15581044
 UltraPure 20X SSC  4 L  15557036
 UltraPure 20X SSPE  4 L  15591035
 UltraPure Acrylamide  500 g  15512023
 UltraPure Agarose & SYBR® Safe DNA Gel Stain Combo  80 applications  15510100
 UltraPure Calf Thymus DNA Solution  5 x 1 mL  15633019
 UltraPure Cesium Chloride, Optical Grade  1 kg  15507023
 UltraPure DEPC-Treated Water  1 L  750023
 UltraPure Dithiothreitol (DTT)  5 g  15508013
 UltraPure DNase/RNase-Free Distilled Water  10 x 500 mL  10977023
 UltraPure Formamide  500 g  15515026
 UltraPure Glycerol  500 mL  15514011
 UltraPure Guanidine Hydrochloride  500 g  15502016
 UltraPure Guanidine Isothiocyanate  500 g  15535016
 UltraPure Guanidine Isothiocyanate Solution  500 mL  15577018
 UltraPure Urea  500 g  15505035
 UltraPure Urea  2 kg  15505050
 UltraPure Herring Sperm DNA Solution  5 x 1 mL  15634017
 UltraPure Salmon Sperm DNA Solution  5 x 1 mL  15632011
 UltraPure TEMED  30 mL  15524010
 UltraPure Tris  1 kg  15504020
 50X Denhardt's Solution  100 ml  750018

For Research Use Only. Not for use in diagnostic procedures.