RiboMinus™ technology is designed to enrich the whole spectrum of RNA transcripts by selectively depleting ribosomal RNA molecules (rRNA), regardless of their polyadenylation status or the presence of of a 5'-cap structure. The RiboMinus™ method has been shown to remove the vast majority of the most abundant ribosomal RNA molecules (up to 99.9%) to allow for greater interrogation of less abundant transcripts.
Working with something other than human, mouse, or plant? Contact us with your species and sequence. Simply include your FASTA sequence in *.txt file format for the ribosomal sequences you would like to verify, with the subject line titled “RiboMinus for (your organism here)”, and we'll work with you to find the right probe for your RiboMinus® rRNA depletion.
The protocol begins with total RNA purified from culture, tissues, or blood using a method of choice (e.g., PureLink™ RNA Mini Kit, TRIzol® Reagent)
The total RNA is then hybridized with Locked Nucleic Acid (LNA) probes specific for abundant ribosomal RNA molecules.
Next, the unwanted rRNA are separated from the RiboMinus™- enriched complexes using RiboMinus™ Magnetic beads.
The RiboMinus™-enriched RNA sample is then concentrated using ethanol precipitation or a silica spin column step.
The enriched RNA fraction is now ready for downstream processing (e.g., microarray, RNA-Seq).