Ambion® RNA experts are committed to providing the highest-quality prepared RNA available. To achieve this goal we are continually striving to understand what "high-quality RNA" truly means with respect to various downstream applications. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects of RNA quality. As we continue to gain a better understanding of how these factors affect downstream applications, we will strive to ensure that our FirstChoice® RNA products exceed the requirements of even the most stringent applications.

  • Certified to contain small RNAs (miRNA, siRNA, and snRNA)
  • RNA available from a wide variety of human tissues and cell lines
  • DNase-treated to remove contaminating DNA
  • Superior quality control standards

Total RNA

We offer FirstChoice® total RNA from human, mouse, and rat tissues, as well as from E. coli and human cell lines. RNA is obtained using Ambion® RNA isolation reagents to yield highly pure, intact RNA that includes the small-RNA fraction. Purified RNA is subjected to a stringent DNase treatment. The RNA is supplied ready to use in any downstream application, including RT-PCR. Note that although it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant.

RNA integrity is verified by capillary electrophoresis using an Agilent® 2100 Bioanalyzer™ instrument. FirstChoice® total RNA is provided in THE RNA Storage Solution at a convenient, ready-to-use concentration of 1 mg/mL.

Ordering Information

Product Name Cat. No. Species Sample Source Quantity
E. coli Total RNA AM7940 E. coli Normal Tissue 200 µg
Breast Adenocarcinoma (MCF-7) Total RNA AM7846 Human Cell line 100 µg
Burkitts Lymphoma (Raji) Total RNA AM7856 Human Cell line 100 µg
Cervical Adenocarcinoma (HeLa-S3) Total RNA AM7852 Human Cell line 100 µg
FirstChoice® Human Brain Reference RNA (1 mg⁄mL) AM6050 Human Normal tissue 50 µg
Human Brain Total RNA AM7962 Human Normal tissue 100 µg
Human Breast Total RNA AM6952 Human Normal tissue 50 µg
Human Colon Total RNA AM7986 Human Normal tissue 100 µg
Human Heart Total RNA AM7966 Human Normal tissue 100 µg
Human Kidney Total RNA AM7976 Human Normal tissue 100 µg
Human Liver Total RNA AM7960 Human Normal tissue 100 µg
Human Lung Total RNA AM7968 Human Normal tissue 100 µg
Human Pancreas Total RNA AM7954 Human Normal tissue 100 µg
Human Prostate Total RNA AM7988 Human Normal tissue 100 µg
Human Skeletal Muscle Total RNA AM7982 Human Normal tissue 100 µg
Human Spleen Total RNA AM7970 Human Normal tissue 100 µg
Human Testes Total RNA AM7972 Human Normal tissue 10 µg
Leukemia (K-562) Total RNA AM7832 Human Cell line 100 µg
Promyelocytic Leukemia (HL-60) Total RNA AM7836 Human Cell line 100 µg
T-cell Leukemia (Jurkat) Total RNA AM7858 Human Cell line 100 µg

NOTE: Ambion® no longer sells the following purified RNAs/cDNAs as catalog made-to-stock products:

  • FirstChoice® Tumor/Normal Adjacent Tissue RNA
  • FirstChoice® Brain Region RNA
  • FirstChoice® Rat/Mouse RNA
  • FirstChoice® Poly(A) RNA
  • FirstChoice® cDNA (either PCR-ready or RACE-ready)

 

To order any of the above products please contact customer service.