Ambion® RNA experts are committed to providing the highest-quality prepared RNA available. To achieve this goal we are continually striving to understand what "high-quality RNA" truly means with respect to various downstream applications. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects of RNA quality. As we continue to gain a better understanding of how these factors affect downstream applications, we will strive to ensure that our FirstChoice® RNA products exceed the requirements of even the most stringent applications.
- Certified to contain small RNAs (miRNA, siRNA, and snRNA)
- RNA available from a wide variety of human tissues and cell lines
- DNase-treated to remove contaminating DNA
- Superior quality control standards
We offer FirstChoice® total RNA from human, mouse, and rat tissues, as well as from E. coli and human cell lines. RNA is obtained using Ambion® RNA isolation reagents to yield highly pure, intact RNA that includes the small-RNA fraction. Purified RNA is subjected to a stringent DNase treatment. The RNA is supplied ready to use in any downstream application, including RT-PCR. Note that although it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant.
RNA integrity is verified by capillary electrophoresis using an Agilent® 2100 Bioanalyzer™ instrument. FirstChoice® total RNA is provided in THE RNA Storage Solution at a convenient, ready-to-use concentration of 1 mg/mL.
NOTE: Ambion® no longer sells the following purified RNAs/cDNAs as catalog made-to-stock products:
- FirstChoice® Tumor/Normal Adjacent Tissue RNA
- FirstChoice® Brain Region RNA
- FirstChoice® Rat/Mouse RNA
- FirstChoice® Poly(A) RNA
- FirstChoice® cDNA (either PCR-ready or RACE-ready)
To order any of the above products please contact customer service.