Laser capture microdissection technique
Laser capture microdissection (LCM) is an automated sample preparation technique that enables isolation of specific cells from a mixed population under microscopic visualization. This technique of isolating a pure sample from a heterogeneous mixture allows for more efficient and accurate results with downstream microgenomics applications such as next-generation sequencing, Sanger sequencing, PCR, and proteomics.
The isolation and characterization of specific cells from a heterogeneous cell population is important for the analysis of gene expression because the differences between various cell types present within normal tissues have significant effects on gene expression analysis. LCM allows one to obtain answers in a single cell that may otherwise have been missed in a whole tissue scrape that contains a heterogeneous mixture of healthy, stromal and cancer cells.
The LCM technique can be used to isolate just a few cells or even a single cell from tissue samples, blood, or even semen samples, depending on the application. The cells can be chosen on the basis of their morphology, immunohistochemical phenotype, or even their genotype, using in situ hybridization methods.
LCM is designed to be precise, contact-free and to help minimize contamination, to uncover answers that might otherwise be missed using a nontargeted sample due to the small size or homogenized nature of the sample. The images below show the isolation of a cell population using the LCM technique.
Compare laser capture microdissection and laser microdissection (LMD) methods
The ArcturusXT™ LCM system is for research use only, and is not intended for animal or human therapeutic or diagnostic use.