Analytical Filtration

Reliable and consistent analytical filterware for microbiological quality control testing

When conducting analytical filtration in your lab, we understand that particle retention efficiency is critical to your research. Analytical filter units and filter funnels are often used in water quality testing to determine the microbial content of samples. Producing accurate results in this type of testing is especially dependent on the filter’s performance in retaining microbes on the membrane.

We built our Nalgene analytical filterware products with all of this in mind. For over 50 years, we have provided precise, easy-to-use systems for the recovery of microorganisms and particles for QC testing and research. We provide sterile analytical filters, funnels and a wide array of reusable filters to support a variety of analytical filtration applications. 

The most common applications for Nalgene Analytical Filtration products are for the Water Quality, Food and Beverage, and Pharmaceutical industries.

Water quality
  • Waste Water
  • Potable Water
  • Recreational Water
    (Beaches, Lakes, etc)
  • Swimming Pools
Food & beverage
  • Wine
  • Beer
  • Milk
  • Packaged Foods
  • Fruit Juices
Pharmaceutical
  • Raw Ingredients
    (liquid and powder)
  • Make-up Water
  • Rinse Water
  • Final Product

Nalgene analytical system

analytical workflow

Select a membrane by color and pore size:

Color
White
  • Standard for microbiological analysis of water, wastewater, and beverage
Grey
  • For the maximum contrast between colonies and the filter without staining
  • Use to enumerate yeast and bacteria in beverages, wines, wastewater, and water
Pore size
Challenge organism
0.2μm Small bacteria
0.45μm Coliforms, fecal coliforms, Common bacteria
0.8μm Yeasts, molds and fungi
1.0μm Yeasts, molds and fungi

Select a filter unit or manifold assembly

Filter Unit
Manifold or Vacuum Flask + Filter Funnel Bottle Top Filter + Receiver Bottle

  • Usable in the field
  • Self contained and ready to use
  • Retains filter samples for other tests
  • Convenient when test many samples at the same time 
  • Reduces waste
  • Can process 3-6 samples at time on one manifold
  • Reduced cost per test completed
  • Reusable funnels and disposable/single-use funnels
  • Reduces waste 
  • Usable in the field
  • Self-contained
  • Reduced cost per test completed

Single use 

Reusable 

Filter Funnel Forceps
Vacuum Flask  Vacuum Manifold

→ Attach Vacuum pump

 
Dilute Sample
(as required by protocol)
As collected 
(no dilution)

1:1 with sterile fluid

1:90 with sterile fluid

1:99 with sterile fluid
Add Sample Remove dust cover Pour sample into upper chamber
Apply Vacuum Open vacuum source, slowly Allow sample to filter through completely Close vacuum
Rinse Funnel
(as required by protocol)
Rinse sides of funnel with sterile fluid very gently
 (do not touch membrane surface)

Open vacuum

 

Allow rinse to filter through completely Close vacuum
Open Unit Gently remove upper funnel section of unit
Retrieve Membrane Gently slip forceps under membrane and lift slowly from supporting surface
Plate membrane on media Place membrane onto prepared medium 
Incubate & retrieve plates Cover plated membrane and place in incubator, remove the plates from incubator after required time
Examine Plated Membrane Check for growth of colonies of correct color and shape
Count Colonies Count colonies per protocol and multiply counts by dilution factor (if diluted)
Discard plates

Select a membrane by color and pore size:

Color
White
  • Standard for microbiological analysis of water, wastewater, and beverage
Grey
  • For the maximum contrast between colonies and the filter without staining
  • Use to enumerate yeast and bacteria in beverages, wines, wastewater, and water
Pore size
Challenge organism
0.2μm Small bacteria
0.45μm Coliforms, fecal coliforms, Common bacteria
0.8μm Yeasts, molds and fungi
1.0μm Yeasts, molds and fungi

Select a filter unit or manifold assembly

Filter Unit
Manifold or Vacuum Flask + Filter Funnel Bottle Top Filter + Receiver Bottle

  • Usable in the field
  • Self contained and ready to use
  • Retains filter samples for other tests
  • Convenient when test many samples at the same time 
  • Reduces waste
  • Can process 3-6 samples at time on one manifold
  • Reduced cost per test completed
  • Reusable funnels and disposable/single-use funnels
  • Reduces waste 
  • Usable in the field
  • Self-contained
  • Reduced cost per test completed
Dilute Sample
(as required by protocol)
As collected 
(no dilution)

1:1 with sterile fluid

1:90 with sterile fluid

1:99 with sterile fluid
Add Sample Remove dust cover Pour sample into upper chamber
Apply Vacuum Open vacuum source, slowly Allow sample to filter through completely Close vacuum
Rinse Funnel
(as required by protocol)
Rinse sides of funnel with sterile fluid very gently
 (do not touch membrane surface)

Open vacuum

 

Allow rinse to filter through completely Close vacuum
Open Unit Gently remove upper funnel section of unit
Retrieve Membrane Gently slip forceps under membrane and lift slowly from supporting surface
Plate membrane on media Place membrane onto prepared medium 
Incubate & retrieve plates Cover plated membrane and place in incubator, remove the plates from incubator after required time
Examine Plated Membrane Check for growth of colonies of correct color and shape
Count Colonies Count colonies per protocol and multiply counts by dilution factor (if diluted)
Discard plates

Setting the industry standard

ISO tested & approved

The Thermo Scientific Nalgene Analytical Filter Units and Filter Funnels with cellulose nitrate membranes exceed the microbial recovery requirements set forth in ISO 7704. Their performance in this application makes them an excellent choice for use in water quality testing.  

APHA standard & US EPA

The CN membranes in Nalgene analytical filter products are manufactured for use in the bacteriological analysis of potable waters in accordance with procedures referenced in APHA Standard Method and laboratory standards for equipment and materials set forth by the U.S. Environmental Protection Agency. 

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