Try our oligo calculator to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method.

Resuspension calculator

This calculates the volume needed to resuspend an oligo, given that nmoles and desired concentration are known.

TermNumber
nmol Oligo Yield (from COA)
Desired conc. (µM or µmol/L)
Oligo Yield (from COA)umol
Volume Required (mL)milliliters
Volume Required (µL)...

Estimated percentage of full length product calculator

This calculates the estimated percentage of full length product based on the oligo's length.

TermNumber
Oligo length (bp)
Chem. reaction efficiency
(typically 98%-99% efficient)
99%
% Full length product...

Minimum yield guarantee oligo calculator

This calculates the minimum yield guaranteed* based on the oligo length (bp) and synthesis scale.

TermNumber
Oligo length (bp)
Approx. mol. weight
(daltons or g/mol)
...
pmol in 1 µg of primer...

Minimum amount (nmoles) delivered

The amounts calculated in the table below represent the minimum guaranteed amount of oligo you will receive based on the oligo length (bp) and synthesis scale.

Synthesis scalesDesalted (nmol)Cartridge (nmol)HPLC (nmol)PAGE (nmol)
25 nmolN/AN/AN/A
50 nmol
200 nmol
1000 nmol (1 µmol)
10000 nmol (10 µmol)N/A

Minimum amount (µL) delivered

The volumes calculated in the table below represent the minimum guaranteed amount of oligo you will receive based on the oligo length (bp), synthesis scale, and selected concentration.

Selected conc.Synthesis scalesDesalted (µL)Cartridge (µL)HPLC (µL)PAGE (µL)
10 µM25 nmolN/AN/AN/A
50 nmol
200 nmol
1 µmol
10 µmolN/A
20 µM25 nmolN/AN/AN/A
50 nmol
200 nmol
1 µmol
10 µmolN/A
50 µM25 nmolN/AN/AN/A
50 nmol
200 nmol
1 µmol
10 µmolN/A
100 µM25 nmolN/AN/AN/A
50 nmol
200 nmol
1 µmol
10 µmolN/A
200 µM25 nmolN/AN/AN/A
50 nmol
200 nmol
1 µmol
10 µmolNA

* Minimum yields guaranteed – when ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonecleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Invitrogen guarantees the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.