PCR Master Mixes

We offer a wide selection of high-quality PCR enzymes and reagents to meet your research needs. Choose from a variety of enzyme application types, formats, buffers, and dNTP solutions depending on the flexibility needed to perform your experiments. With PCR enzymes you know and trust such as AmpliTaq™, AmpliTaq Gold™, Platinum™ Taq, AccuPrime™, and Platinum™ SuperFi™ DNA polymerases, we have what you need for successful PCR.

PCR Types

For everyday PCR amplification
  Taq DNA polymerase
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AmpliTaq™ DNA polymerase
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Amplicon size Up to 5 kb Up to 5 kb
Activation time Immediate Immediate
Product overhang 3′ A 3′ A
Exonuclease activity 5′→ 3′ 5′→ 3′
Master mix/SuperMix format Colorless  
Stand-alone enzyme

Colorless
(Recombinant)
Colorless
(Native)*

Colorless

*Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance inPCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified.

Other PCR enzymes

You can also use these enzymes for everyday PCR amplification:

To increase specificity and yield
  Platinum™ Taq DNA polymerase
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AmpliTaq Gold™ DNA polymerase
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AmpliTaq Gold™ 360 DNA polymerase
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Hot-start technology Antibody Chemical Chemical
Enhanced specificity
Amplicon size Up to 5 kb Up to 5 kb Up to 5 kb
GC-rich format  
Activation time 30 sec to 2 min 10 min 5-10 min
Master mix/Super mix format Colorless
Green*
  Colorless
Stand-alone enzyme Colorless
Green*
Colorless Colorless

* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Other hot-start PCR enzymes

For when sequence accuracy is critical
  Platinum SuperFi DNA Polymerase
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AccuPrime Taq DNA Polymerase High Fidelity
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Platinum Taq DNA Polymerase High Fidelity
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Fidelity >100x 9x 6x
Hot-start for enhanced specificity
Amplicon size up to 20 kb* up to 20 kb up to 20 kb
Overhang Blunt 3′ A/Blunt 3′ A/Blunt

GC-rich format    
Master mix/super mix format Colorless
Green**
  Colorless
Stand-alone enzyme Colorless
Green**
Colorless Colorless

* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Other high-fidelity PCR enzymes

For robust yields of templates longer than 10 kb
  Platinum™ SuperFi™ DNA Polymerase
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Elongase™ Enzyme Mix 
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Amplicon size Up to 20 kb* Up to 30 kb
Fidelity >100x 5x
Hot-start for enhanced specificity  
Master mix/SuperMix Colorless
Green**
 
Stand-alone enzyme Colorless
Green**
Colorless

* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify multiple targets in one reaction
  AccuPrime™ Taq DNA Polymerase System
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Platinum™ SuperFi™ DNA Polymerase
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Platinum™ Multiplex PCR Master Mix
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No. of amplicons in single reaction Up to 15-plex Up to 15-plex Up to 20-plex
Amplicon size Up to 5 kb Up to 2.5 kb Up to 2.5 kb
Hot-start for enhanced specificity
Master mix/super mix format Colorless (SuperMix I)
Colorless (SuperMix II)
Colorless
Green*
Colorless
Stand-alone enzyme Colorless Colorless
Green*
 

* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Other reagents for multiplex PCR

Videos
What is hot-start PCR?
What is high-fidelity PCR?

Resources

Tm Calculator
Use our application to calculate the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases.

Molecular Biology Webinar Series
View our webinar series to learn more about molecular biology topics such as reverse transcription, PCR and cloning.

Video Library
Watch videos from our collection to learn more about PCR.

Mobile & Desktop Apps
Access scientific content and helpful tools through gartis, PCR Essentials and Cloning Bench mobile apps.

PCR Protocols
Browse our technical reference library of PCR protocols.

Support

PCR and cDNA Synthesis Support Center
Find tips, troubleshooting help, and resources for your end-point PCR and cDNA applications. 

Product Support Documentation
Search for manuals, protocols, Material Safety Data Sheets, product literature and certificates by catalog number or product name.

Contact Us
Email or call our Technical Application Scientists for additional questions regarding PCR enzymes and master mixes.