SNP genotyping using 48-, 96-, and 384-well real-time PCR instruments

TaqMan SNP Genotyping Assays use TaqMan 5´ nuclease assay chemistry to amplify and detect specific SNP alleles in purified genomic DNA samples. Each assay allows genotyping of individuals for a specific SNP.

  • World’s largest collection of single-tube, ready-to-use SNP assays available
  • Robust custom assay design pipeline enables highly accurate, reproducible, and reliable results
  • Easy ordering and a simple workflow enable quick results
  • Suggested panels to get you started quickly for many applications
  • Superior Applied Biosystems real-time PCR systems available to meet all throughput needs and budgets for processing and analyzing <1,000 samples a day 

Experimental workflow for SNP genotyping (human and animal)

For plant samples, please visit our Agrigenomics section.

We provide multiple solutions to isolate and purify DNA.

DNA Extract All Reagents Kit
The DNA Extract All Reagents Kit provides PCR-ready DNA from a variety of sample types, ranging from blood to buccal swabs to plant tissue, in 5 minutes.

BloodPrep DNA Chemistry for Cultured Cells and Blood
BloodPrep chemistry is designed to rapidly isolate DNA from fresh or frozen blood of any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the Applied Biosystems 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

Select or design TaqMan probes

We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications.

TaqMan SNP Genotyping Assays
With 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70,000 cSNPs, and 160,000 validated assays, TaqMan SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan chemistry.

Custom TaqMan SNP Genotyping Assays 
Order custom single-tube TaqMan Assays to perform genotyping studies of any possible SNP in any organism. The Custom TaqMan Assay Design Tool allows you to quickly order custom TaqMan Assays by submitting target sequences of interest or custom primer and probe sequences for any organism.

TaqMan Drug Metabolism Assays 
Genotype 2,700 high-value polymorphisms located in regulatory elements and coding regions for 221 drug metabolism and transporter genes.

Prepare a reaction plate and amplify DNA using sequence-specific primers and a TaqMan® probe on an Applied Biosystems real-time PCR system or thermal cycler.

Reagents & kits

TaqPath ProAmp Master Mixes*
TaqPath ProAmp Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mix includes ROX™ passive reference dye for lower multiplexing of up to 3 targets.

TaqPath ProAmp Multiplex Master Mix*
TaqPath ProAmp Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Multiplex Master Mix includes MUSTANG PURPLE™ passive reference dye for higher multiplexing of up to 4 targets.

* General purpose reagents: TaqPath master mixes are general purpose reagents manufactured in an ISO 13485–certified facility and labeled "For Laboratory Use."

Instruments & accessories

ProFlex PCR System
The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently.

SimpliAmp Thermal Cycler
The Applied Biosystems SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Innovations like a responsive color touch screen and Applied Biosystems VeriFlex temperature control technology enable simple, accurate optimization. 

Perform an endpoint plate read using Applied Biosystems real-time PCR systems; the MBG probes of the Applied Biosystems TaqMan SNP Genotyping Assay provide a fluorescence signal for the amplification of each allele.

Instruments & accessories

QuantStudio 3 and 5 real-time PCR systems
The Applied Biosystems QuantStudio 3 and 5 systems offer the ultimate in connectivity and ease of use. They are designed for both new and experienced users who need simple and affordable real-time PCR systems without compromising performance and quality.

QuantStudio 6 Flex Real-Time PCR System 
The Applied Biosystems QuantStudio 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications, and for users on a limited budget. With a planned upgrade path to a QuantStudio 7 Flex System that accommodates automation or TaqMan® Array Cards, the QuantStudio 6 Flex System is an ideal qPCR platform to accommodate changing future needs.

QuantStudio 7 Flex Real-Time PCR System
The Applied Biosystems QuantStudio 7 Flex Real-Time PCR System delivers the proven reliability, sensitivity, and accuracy of the ViiA 7 System in a new industrial design. The QuantStudio 7 Flex System has been optimized to enable the broadest range of quantitative PCR applications, with additional dyes, formats, and automation options.

QuantStudio 12K Flex Real-Time PCR System
Combining flexible throughput capabilities with a streamlined workflow, the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System takes you from targeted discovery through confirmation and screening, all on a single platform.

The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.

Thermo Fisher Cloud Genotyping App
The Genotyping app includes improved visuals and integrated traces of allelic discrimination plots to allow thorough QC of SNP assays to accurately reflect the true signals versus background noise. Applied Biosystems qPCR Analysis Modules are web browser–based software applications that bring together multiple data sets in one convenient compatible browser on any device (Mac or PC) with analysis speeds up to 10 times faster than our desktop software versions.

TaqMan Genotyper Software
TaqMan Genotyper Software is for use with TaqMan SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96-, and 384-well microplates or OpenArray Genotyping Plates, and offers a state-of-the-art genotype-calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

Citations

* General purpose reagents: TaqPath master mixes are general purpose reagents manufactured in an ISO 13485–certified facility and labeled "For Laboratory Use."