microRNA & Noncoding RNA Analysis Using Real-Time PCR
Noncoding RNA (ncRNA), such as microRNA (miRNA) and other small ncRNAs are the focus of one of the most exciting new areas of molecular biology. More and more experimental evidence demonstrates that they play a central role in how the genome is regulated, and how traits are passed on or eliminated by environmental and genetic factors. Scientists have just begun to understand this natural “gene silencing” mechanism and its role in genomic pathways.
Life Technologies offers quantitative real-time PCR (qPCR) products and other tools specifically for miRNA and ncRNA research, from isolation through discovery, profiling, quantitation, validation, and functional analysis.
The first critical step in the analysis of microRNA (miRNA) is isolation from a biological sample. Most RNA isolation procedures were developed and optimized to recover total RNA or mRNA while ignoring small molecules. Ambion has developed a complete line of kits and reagents for isolating miRNAs from a wide range of cell and tissue types, as well as from blood and FFPE samples. Find the right kit for you needs here.
Profile hundreds of known miRNAs with a simple workflow. Options are available for profiling a few samples per day and for large, high-throughput studies.
Quantitate individual miRNAs from a broad assortment of species, including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis.
Identify gain- and loss-of-function phenotypes of genes and processes regulated by miRNAs.
Measure miRNA “gene” transcription in human, mouse or rat species.
Quantitate any nucleic acid less than 200 bases long, including newly discovered miRNAs, Piwi-interacting RNA (piRNA), small nuclear RNA (snRNA), and small nucleolar RNA (snoRNA).
Quantitate noncoding RNA transcripts longer than 200 bases in human, mouse or rat species.
For Research Use Only. Not for use in diagnostic procedures.