microRNA-Adapted

miRNAs (microRNAs) are short (19–25 nucleotides in length), noncoding RNAs that post-transcriptionally regulate gene expression and control diverse biological processes.  miRNAs have significant promise as biomarkers for diseases, given their regulatory role in many cellular processes.  The inherent value of miRNA profiles in areas of translational research, infectious diseases, reproductive and genetic health, and biomarker discovery and tumor diagnostics has boomed over the past several years, in an attempt to seek out targeted therapies that will improve future health outcomes. 

Over half of the research activity analyzing microRNAs, including research using qPCR, focuses on cancer (based on research publications). The pie chart below is an illustration of common disease applications for miRNA research conducted using real-time PCR. 


Applied Biosystems TaqMan miRNA products provide extensive coverage of the Sanger miRBase registry and offer the flexibility to accomplish your research goals.  The streamlined workflow combined with ultra-high specificity, sensitivity, and dynamic range of TaqMan Assays make these solutions an ideal choice for miRNA profiling from precious, limited samples such as FFPE and biofluids across a range of species.

Features include:

  • Universal reverse transcription (RT)—one streamlined RT step for all TaqMan Advanced miRNA Assays with miRBase v.21 coverage
  • Ultra-sensitive—detect as few as 60 copies of input miRNA
  • Highly specific—detect only mature miRNA and distinguish between highly homologous miRNAs
  • Small sample input—detect and quantify mature miRNA from as little as 1 pg of total RNA or 2 µL of plasma or serum

Stretch your abilities even further with our intuitive qPCR analysis tools. qPCR analysis modules are cloud-based secondary data analysis solutions that combine multiple data sets in one convenient place, making it easier for you to view, store, and analyze your qPCR data. Learn more ›

Featured miRNA profiling solutions using real-time PCR

Comes in a 384-well microfluidic card format that enables accurate quantitation of 192 unique targets in as little as five hours.

Accurately quantitate 754 unique miRNAs in as little as five hours with the convenience of pre-spotted TaqMan Advanced miRNA Assays in a 384-well microfluidic card.

Maximize throughput with minimal resources with a system that takes you from targeted discovery through confirmation and research screening, all on a single platform.


Selection guide: TaqMan miRNA assays for real-time PCR
  TaqMan MicroRNA Assays TaqMan Advanced miRNA Assays
Description Employ a novel target-specific stem-loop primer during cDNA synthesis that produces a template for real-time PCR Employ a universal RT step for a streamlined workflow and a universal miR-Amp step
RT chemistry miRNA-specific RT Universal RT
Throughput Best for 1–10 targets Best for >10 targets
Coverage 205 species available; coverage for miRBase v.21 All human, mouse, and rat miRNAs; coverage for miRBase v.21
Formats Available in individual tubes, TaqMan array cards and plates, and OpenArray formats Available in individual tubes, TaqMan array cards and plates, and OpenArray formats; inquire for custom plating options

Flexible formats
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TaqMan Advanced miRNA single-tube assays
Conserve limited samples; requires only 1–10 ng of total RNA or equivalent, and is ideal for a small number of assays

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New TaqMan Advanced miRNA 96-well plates
Comprehensive—choose from a large collection of pre-plated, predesigned panels to pinpoint your specific target

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New TaqMan Advanced miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 754 miRNA targets from as little as 1 ng of total RNA

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New TaqMan Advanced miRNA OpenArray plates
Run three samples per QuantStudio 12K Flex OpenArray Plate, translating up to 799 assays per sample (up to 752 unique assays)

Find the right microRNA product for your research

Choose from our complete list of miRNA assays and formats using our selector tool.
Learn more ›

Flexible content panels

TaqMan flexible content panels offer an easy way to select and design a combination of assays in the format that matches your experiment. We offer a wide range of flexible content panels.
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Custom assay services

Want us to produce a custom assay for you?
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