TaqMan Mutation Detection Assays
TaqMan™ Mutation Detection Assays are powered by competitive allele-specific TaqMan™ PCR (castPCR™ Technology) to detect and measure somatic mutations in genes associated with cancer research. The castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh frozen tissue samples.
Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.
By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays give you:
- Higher specificity—designed to block the wild-type and amplify only the mutant
- Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
- Faster workflow—3 hours from sample to result
The TaqMan™ Mutation Detection IPC Reagent Kit is a set of optional internal positive control reagents that can be duplexed with any TaqMan Mutation Detection Assay to provide a positive PCR control result. The TaqMan Mutation Detection IPC Reagent Kit is pre-optimized for use with the TaqMan Mutation Detection Assays.
Order your TaqMan Mutation Detection Assays pre-plated into Fast or standard 96- or 384-well plates with the TaqMan™ Custom Plating Service. Save time and reduce the resources needed for large-scale experiments.
TaqMan Assay documentation files
The TaqMan documentation files can be downloaded at thermofisher.com/taqmanfiles
Manuals & Protocols
Protocol: TaqMan Mutation Detection Assays
Quick Reference Card: TaqMan Mutation Detection Assays
User Guide: Mutation Detector™ Software
Quick Reference Card: Mutation Detector™ Software
TaqMan Mutation Detection Assay Index File
|Discrimination||castPCR technology combines allele-specific TaqMan qPCR with allele-specific MGB oligonucleotide blocker to effectively suppress non-specific amplification from the off-target allele.|
|Assay Components||Each pre-formulated mutant or wild type allele assay contains:
A 10X mix of unlabeled PCR primers, a TaqMan™ MGB probe (FAM™ dye-labeled), and an MGB oligonucleotide blocker
Each pre-formulated gene reference assay contains:
A 10X mix of unlabeled PCR primers and a TaqMan MGB probe (FAM™ dye-labeled)
|Reactions||Sufficient for 150 10µl reactions in a 384-well plate or 75 20µl reactions in a 96-well plate.|
|Expiration Date||2 years from the date of manufacture|
|Assay Information File||Includes sales order number, part number, assay ID, NCBI gene name, and entrez gene ID. Also includes Sanger COSMIC ID, nucleotide mutation, amino acid change, and mutation type.|
|Documentation||Available to download at thermofisher.com/taqmanfiles:
|Shipping and Storage||
Shipped at ambient temperature.
|Catalog #||Product Name||Availability|
|4465804||TaqMan Mutation Detection Assays (mutant allele assays and wild type allele assays)||Inventoried|
|4465805||TaqMan™ EGFR Exon 19 Deletions Assay (mutant allele assay to 19 different EGFR exon 19 deletions)||Inventoried|
|4465807||TaqMan Mutation Detection Reference Assays (gene reference assays)||Inventoried|
|4467538||TaqMan Mutation Detection IPC Reagent Kit||Inventoried|
The power of digital PCR can also be used to detect rare mutations for cancer research. Using the QuantStudio™ 3D Digital PCR System and wet-lab validated TaqMan SNP Genotyping Assays, you can target over 100 somatic mutations at a prevalence as low as 0.1%.
For Research Use Only. Not for use in diagnostic procedures.