TaqMan® Mutation Detection Assays detect somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan® PCR technology, also known as castPCR™ technology. The castPCR™ technology is highly specific and sensitive, and it can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA.

  • High specificity—mutant allele detection is based on an allele-specific primer, while the wild-type background is suppressed by an MGB blocker oligonucleotide
  • High sensitivity—assays can detect as low as 0.1% mutant molecules in a background of wild type gDNA as demonstrated in spiking experiments
  • Wide dynamic range and excellent PCR efficiency—assays demonstrate at least 4 logs of dynamic range and an average efficiency of 100 ± 10%
  • Fast, simple workflow—like other TaqMan® Assays, typically requires 3 hours from sample to results, with minimum hands-on time
  • Our current assay set covers 778 assays to key mutation targets in 46 cancer genes

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Discrimination castPCR™ technology combines allele-specific TaqMan® qPCR with allele-specific MGB oligonucleotide blocker to effectively suppress non-specific amplification from the off-target allele.
Assay Components Each pre-formulated mutant or wild type allele assay contains:
A 10X mix of unlabeled PCR primers, a TaqMan® MGB probe (FAM™ dye-labeled), and an MGB oligonucleotide blocker

Each pre-formulated gene reference assay contains:
A 10X mix of unlabeled PCR primers and a TaqMan® MGB probe (FAM™ dye-labeled)
Reactions Sufficient for 150 10µl reactions in a 384-well plate or 75 20µl reactions in a 96-well plate.
Expiration Date 2 years from the date of manufacture
Assay Information File Includes sales order number, part number, assay ID, NCBI gene name, and entrez gene ID. Also includes Sanger COSMIC ID, nucleotide mutation, amino acid change, and mutation type.
Documentation Available to download at thermofisher.com/taqmanfiles :
  • Protocol for TaqMan® Mutation Detection Assays (pdf file)
  • TaqMan® Mutation Detection Assays Quick Reference Card (pdf file)
  • Product Insert (pdf file)
  • Assay Information File (XML and HTML formats)
  • Understanding Your shipment document (pdf file)
  • Safety data sheet (pdf file)
  • Datasheet (pdf file)
  • Certificate of Analysis (pdf file)
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Catalog # Product Name Availability
4465804 TaqMan® Mutation Detection Assays (mutant allele assays and wild type allele assays) Inventoried
4465805 TaqMan® EGFR Exon 19 Deletions Assay (mutant allele assay to 19 different EGFR exon 19 deletions) Inventoried
4465807 TaqMan® Mutation Detection Reference Assays (gene reference assays) Inventoried
4467538 TaqMan® Mutation Detection IPC Reagent Kit Inventoried
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