Real-time PCR

Real-time PCR (also known as quantitative PCR or qPCR) is the simultaneous amplification and detection/quantification of nucleic acids using the polymerase chain reaction (PCR). qPCR is a versatile technique and a powerful tool to support your research applications. Find optimized real-time PCR master mixes, reagents, and kits to power your experiments here.

Want to try a free sample of our best performing master mixes? Visit our qPCR master mix sample request page.

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Recommended qPCR master mixes by application



Start with..



TaqMan™ Fast Advanced Master Mix
for best performance 2-step
TaqMan™ Fast Virus Master Mix  
for 1-step virus detection and gene expression


TaqMan™ Genotyping Master Mix


TaqMan™ Pre-Amp Master Mix
for limited samples (e.g., FFPE)



PowerUp SYBR™ Green Master Mix

(Clinical-grade TaqMan)
General purpose reagent.
For laboratory use.

diagnostics development

TaqPath™ qPCR Master Mix
for DNA detection or 2-step gene expression
TaqPath™ 1-step RT-qPCR Master Mix
TaqPath™ 1-step Multiplex Master Mix
for RNA detection

With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required:

Looking for qPCR reagents for molecular diagnostic test development? 
See our molecular diagnostic section

Looking for qPCR multiplexing solutions? 
See our qPCR multiplex applications section

Real-time PCR master mix selection tool

Select from our full selection of optimized Applied Biosystems™ and Invitrogen™ real-time PCR reagents to find the one that’s right for your desired application, detection chemistry, format, and running mode.

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Application Detection Chemistry Format Mode Recommended SKU Product Qty