Real-time PCR

Real-time PCR (also known as quantitative PCR or qPCR) is the simultaneous amplification and detection/quantification of nucleic acids using the polymerase chain reaction (PCR). qPCR is a versatile technique and a powerful tool to support your research applications. Find optimized real-time PCR master mixes, reagents, and kits to power your experiments here.

Want to try a free sample of our best performing master mixes? Visit our qPCR master mix sampling Web page.

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qPCR master mixes by application

1-step RT-qPCR master mixes by application

Chemistry Application Recommended product
TaqMan Gene expression analysis and virus detection TaqMan™ Fast Virus Master Mix
Molecular diagnostics development TaqPath™ 1-Step RT-qPCR Master Mix, CG
General purpose reagent. For laboratory use.
Mulitplexing (GEx, virus detection and molecular Dx development) TaqPath™ 1-Step Multiplex Master Mix
General purpose reagent. For laboratory use.
SYBR 1-step gene expression analysis Power SYBR™ Green RNA-to-Ct™ 1-Step Kit
  Good results for gene expression Optimized for broad qPCR applications Convenient and fast
Starting template DNADNARNA
ApplicationsGene expression
DNA quantitation (pathogen detection)
CHiP
Gene expression
DNA quantitation
CHiP
SNP genotyping
microRNA & small RNAs
Copy number variation
Pathway analysis
Mutation detection
Protein analysis
Multiplexing
Virus detection and quantification
High-throughput gene expression
High-throughput screening
RNA quantification
Primer design requiredyes  
RT required,
learn more >
yesyes 
Resources 
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Sample-to-answer
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required:


Looking for qPCR reagents for molecular diagnostic test development? 
See our molecular diagnostic section


Looking for qPCR multiplexing solutions? 
See our qPCR multiplex applications section


Real-time PCR master mix selection tool

Simple and easy! To identify the optimized Applied Biosystems™ or Invitrogen™ real-time PCR reagent that’s right for you, find your desired application, detection chemistry, format and running mode.

Please select an Application

  • Application
  • Detection Chemistry
  • Format
  • Mode
  • Recommended
Application Detection Chemistry Format Mode Recommended SKU Product Qty