SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR Green dye—everything needed for real-time PCR, except the sample and PCR primer pair.
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Which SYBR Green master mix is right for you?

Applications Gene Expression Analysis
DNA quantitation (pathogen detection)
Gene Expression Analysis
DNA quantitation (pathogen detection)
Dual-lock for exceptional specificity
Cycling Time Standard (60 minutes) or
Fast (30 minutes)
Standard (60 minutes)
Carryover contamination control Includes heat-labile UNG and a blend of dTTP/dUTP Contains dUTP that is compatible with AmpErase™ UNG (available separately)
72 h stability of reaction mixes prior to qPCRyes  
72 h stability of product
post PCR
Sensitivity 1 copy 2 copies
Instrument compatibilityCompatible with all Applied Biosystems™ real-time PCR instruments, Bio-Rad IQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene™ Mx3005P™ systems Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad IQ5, Roche LightCycler 480, and Stratagene Mx3005P systems
Primer recommendation150–400 nM each
(300–800 nM total)
50–900 nM
Price / 20 µL Reaction $0.63-$1.03 $0.60-$0.98
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