TaqMan® Master Mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable TaqMan® Assay-based real-time PCR. Just add your sample and TaqMan® Assay, and start your reactions.

See all TaqMan® master mixes ›


Which TaqMan® master mix is right for you?

Gene Expression (Standard mode) Gene Expression (Fast mode) Genotyping (Standard mode) Genotyping (Fast mode)
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  TaqMan® Gene Expression Master Mix TaqMan® Fast Advanced Master Mix TaqMan® Genotyping MasterMix TaqMan® GTXpress™ Master Mix
Application examples Gene expression for mRNA Gene expression for mRNA & small RNA SNP genotyping,
Mutation detection,
SNP genotyping, Crude lysate samples
Cycling/Run Time 1:20 0:40 1:20 0:50
UDG to prevent DNA carryover contamination
ROX reference dye to normalize signal & ensure data integrity
Universal cycling for assay to assay consistency
Benchtop stability 24 hr 72 hr 24 hr 24 hr
Tolerant to PCR inhibitors
Price / 20 µL Reaction $0.62-$0.79$0.81-$0.95 $0.58-0.85 $0.60-0.78

*While TaqMan® Universal Master Mix II can be used for all applications, TaqMan® Genotyping Master Mix has been specifically designed and will provide the best performance for genotyping applications (e.g. CNV, DME & SNP).

Other master mixes

TaqMan® PreAmp Master Mix is designed to facilitate analysis of limited-quantity samples, including single cells. Use the mix to expand small amounts of cDNA by pre-amplification without introducing amplification bias to the sample. Pre-amplification enables you to stretch your precious sample for use in many more real-time PCR reactions than you could otherwise run.

Applied Biosystems® TaqMan® Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this master mix to analyze environmental, food, and other challenging samples.

TaqMan® Genotyping Master Mix is specifically formulated for detection of copy number, DME’s, and SNPs. Use this mix for the best performance for genotyping applications.

TaqMan® Fast Environmental Master Mix Beads have been designed to match or exceed the performance of standard (not fast) real-time PCR master mixes, delivering shorter run times than these master mixes (<40 minutes in fast mode) with an easy-to-use, lyophilized bead format. These beads are available with or without a VIC® dye-labeled internal positive control (IPC).

For testing of Food and Environmental samples only.