A simple solution for dengue, chikungunya, and Zika virus RNA detection: just add RNA

Zika virus is a single-stranded RNA arbovirus that is spread to humans primarily through the bite of an infected Aedes-species mosquito, causing Zika virus disease. Infected individuals may have fever, rash, joint pain, and/or conjunctivitis, but symptoms are usually mild. A pregnant woman can pass the virus to her unborn baby, and there may be a link between Zika virus and birth defects.

Current outbreak of Zika virus

In May 2015, the Pan American Health Organization (PAHO) issued an alert regarding the first confirmed Zika virus infection in Brazil, and on February 1, 2016, the World Health Organization declared Zika virus a public health emergency of international concern. Local transmission has been reported in many other countries and territories globally. Zika virus will likely continue to spread (Centers for Disease Control and Prevention, 2016).

For more up-to-date information on Zika virus and the current outbreak, visit the CDC website.

Detection of Zika and other arboviruses

Countries with local transmission of Zika virus often experience transmission of other arboviruses that can cause similar symptoms. Therefore, it is important to test for other common arboviruses simultaneously, such as dengue and chikungunya. Although chikungunya, dengue, and Zika viruses can be detected by testing serum for the presence of viral nucleic acid or virus-specific immunoglobulin M (IgM) and neutralizing antibodies, serological cross-reactivity is strong between Zika and dengue viruses, so molecular detection using real-time PCR is strongly recommended. Thermo Fisher Scientific has worked diligently to develop both a lyophilized one-step real-time PCR multiplex Applied Biosystems™ TaqMan® assay for molecular detection of RNA from the dengue virus (DENV), chikungunya virus (CHIKV), and Zika (ZIKV) virus, and a singleplex Zika virus test.

See the CDC’s updated information on diagnostic testing for Zika, chikugunya, and dengue viruses in US public health laboratories.

Real-time PCR detection of Zika and Arboviruses

Thermo Fisher Scientific offers three types of Zika and Arbovirus real-time PCR solutions:

  • A singeplex Zika virus kit (TaqMan® and TaqPath™ Zika Virus Kits, ZIKV)
  • A triplex Arboviruses kit (TaqPath™ Zika Virus Triplex Kit, ZIKV/DENV/CHIKV)
  • A triplex Zika virus vector screening kit (TaqMan® Zika Virus Triplex Vector Screening Kit, ZIKV/DENV/CHKV)

Features of our singleplex and triplex TaqMan assays for Zika and arboviruses:

  • Lyophilized with one-step RT-PCR master mix; simply add RNA
  • One-step qRT-PCR reaction runs in fast or standard mode
  • Optimized for the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System; also suitable for the 7500, 7500 Fast, and other QuantStudio™ systems
  • Simple workflow for dengue, chikungunya, and Zika virus RNA detection using a triplex TaqMan Assay
  • Easy to use, environmentally friendly, no cold-chain management required, ship and store at room temperature

The Zika virus sequence used to develop these primers and probes was identified in a 2014 research paper by Pyke et al., titled “Imported Zika Virus Infection from the Cook Islands into Australia, 2014” (view full paper).

For viral RNA purification, we recommend use of the MagMAX™ Pathogen RNA/DNA Kit.

Read our Accelerating Science blog “Battling Zika – Do we have your attention now?".

Protocols:
TaqPath Zika Virus Kit (ZIKV), Fast, 0.1 mL block
TaqMan Zika Virus Kit (ZIKV), Standard, 0.2 mL block

How to order, and additional information

To get a quote, or if you would like a sales representative to contact you, please fill the form below.

Cat no. Description Format
Singleplex assay format
A31744 TaqMan Zika Virus Kit (ZIKV) Standard, 0.2 mL block
A31745 TaqPath Zika Virus Kit (ZIKV) Fast, 0.1 mL block
Triplex assay format
A31746 TaqMan Zika Virus Triplex Kit (ZIKV/DENV/CHIKV) Standard, 0.2 mL block
A31747 TaqPath Zika Virus Triplex Kit (ZIKV/DENV/CHIKV) Fast, 0.1 mL block
Vector screening
A26105 TaqMan Zika Virus Triplex Vector Screening Kit (ZIKV/DENV/CHIKV) Standard, 0.2 mL block
A31748 TaqMan Zika Virus Triplex Vector Screening Kit (ZIKV/DENV/CHIKV) Fast, 0.1 mL block
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 TaqMan Zika Virus Kit (0.2 mL, 12 x 8-well strip tubes)
 TaqPath Zika Virus Kit (0.1 mL, 12 x 8-well strip tubes)
 TaqMan Zika Virus Triplex Kit (0.2 mL, 12 x 8-well strip tubes)
 TaqPath Zika Virus Triplex Kit (0.1 mL, 12 x 8-well strip tubes)
 TaqMan Zika Virus Triplex Vector Screening Kit (0.2 mL, 12 x 8-well strip tubes)
 TaqMan Zika Virus Triplex Vector Screening Kit (0.1 mL, 12 x 8-well strip tubes)
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Related products for arbovirus (Zika) detection by real-time PCR

Isolate viral RNA

The Applied Biosystems MagMAX Pathogen RNA/DNA Kit enables purification of RNA and DNA from viruses and easy-to-lyse bacteria and parasites using magnetic particle technology in a 96-well format. PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.

Perform real-time PCR

The QuantStudio 5 Real-Time PCR System is ideal for all levels of users who need a high-performance instrument with features for maximum control of their experiments. Includes built-in security features, locked workflows, user account management, and options for Web browser–based or desktop analysis.


Fully optimized for fast cycling, the 7500 Fast Real-Time PCR System delivers high-quality results in as little as 30 minutes.