Reverse Transcription

We offer a selection of high-quality products for first-strand cDNA synthesis to meet your research needs. Choose from a variety of reverse transcription enzymes, formats, primers, and dNTP solutions that work best for your application. With our premium line of Invitrogen SuperScript Reverse Transcriptases, we have what you need for superior cDNA synthesis results with any type of template RNA.


Reverse transcription products

Stand-alone enzymes for maximum flexibility in reaction setup

  Superior enzyme for all RNAs, including difficult templates Value enzyme for commonly used RNA templates
  Superscript IV Reverse Transcriptase M-MLV-Reverse Transcriptase
Optimal reaction temp 50–55°C 37°C
Reaction time 10 min 50 min
cDNA yield Superior Average
Sensitivity Superior Average
Max cDNA length Up to 12 kb Up to 7 kb
Ability to work with degraded or inhibitor-containing RNA High Low
Reduced RNase H activity Yes No
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Complete kits with all cDNA synthesis reaction components

  Superior choice for all RNAs, including difficult templates Value choice for commonly used RNA templates
  SuperScript IV
First-Strand Synthesis System
High-Capacity cDNA Reverse Transcription Kit
Reverse transcriptase SuperScript IV MultiScribe MuLV
Optimal reaction temp 50–55°C 37°C
Reaction time 10 min 50 min
cDNA yield Superior Average
Sensitivity Superior Average
Max cDNA length Up to 12 kb Up to 7 kb
Ability to work with degraded or inhibitor-containing RNA High Low
Reduced RNase H activity Yes No
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Other cDNA synthesis kits for endpoint PCR applications

cDNA synthesis kits for the highest convenience in RT-qPCR setup

  Superior master mix for all RNA templates Superior master mix for all RNA templates, with fast and easy gDNA removal Value choice for commonly used RNA templates
  SuperScript IV VILO Master Mix SuperScript IV VILO Master Mix with ezDNase Enzyme High-Capacity cDNA Reverse Transcription Kit
Reverse transcriptase SuperScript IV SuperScript IV MultiScribe MuLV
Optimal reaction temp 50°C 50°C 37°C
Reaction time 10 min 10 min 120 min
Ability to work with degraded or inhibitor-containing RNA High High Low
Reduced RNase H activity Yes Yes No
Primers included Random primers & oligo(dT)20 Random primers & oligo(dT)20 Random primers
Integrated gDNA removal No Yes No
Format All RT reaction components premixed All RT reaction components premixed RT reaction components in separate tubes
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One-step RT-PCR kits for fast and simple workflow

  High-performing choice for high-fidelity applications High-performing choice for standard applications
  SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
Reverse transcriptase SuperScript III SuperScript III
PCR enzyme Platinum Taq High Fidelity DNA Polymerase Platinum Taq DNA Polymerase
Optimal reaction temp 55°C 55°C
Reaction time 30 min 30 min
Sensitivity High High
Maximum target length Up to 10 kb Up to 4.5 kb
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Featured videos
Reverse transcriptase reaction in 10 minutes — enzyme processivity
Inhibitors in reverse transcriptase reaction
Understanding reverse transcriptase — Effects on Ct Value
Overcoming RT-qPCR challenges

Resources

Education

Review educational resources for reverse transcription basics, cDNA synthesis, enzyme selection, troubleshooting tips, and cDNA applications.

Molecular Biology Webinar Series

View our webinar series to learn more about molecular biology topics such as reverse transcription, PCR, and cloning.

Mobile & Desktop Apps

Access scientific content and helpful tools through artis and Cloning Bench mobile apps.

OEM & customized cDNA solutions

Tailor-made performance tested products with a strict quality standards  to meet your assay needs.

Support

PCR and cDNA Synthesis Support Center

Find tips, troubleshooting help, and resources for your endpoint PCR and cDNA applications.

Product Support Documentation

Search for manuals, protocols, Material Safety Data Sheets, product literature, and certificates by catalog number or product name.

Contact Us

Email or call our technical application scientists for additional questions regarding PCR enzymes and master mixes.