Reverse transcription is the synthesis of single-stranded DNA (complementary DNA, or cDNA) using single-stranded RNA as a template, mediated by reverse transcriptases (RTs). The cDNA can be used as a template for amplification by PCR or to generate a cDNA library.
Choosing the correct reverse transcriptase for cDNA synthesis is critical to obtaining high yields of quality, full-length cDNA with full gene representation. With thousands of citations, the SuperScript™ family of RTs is a first choice for cDNA synthesis. Use the overview of RTs and cDNA synthesis kit products to choose the solution that’s right for you.