ProFlex PCR System

Flexible configuration and control

Now cloud-enabled for secure remote access

The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. And now you can access your ProFlex PCR System with Thermo Fisher Connect, giving you the freedom to design and securely upload your methods and monitor runs from any smartphone or desktop computer.

Simple to set up
Simple to use

Run three experiments at once with three independently controlled blocks

The ProFlex PCR System with the 3 x 32-well block option can run three different cycling conditions, at three different times by one or multiple users. Additionally, with Thermo Fisher Connect, users can remotely monitor runs from their smartphones or desktop computers. These features make the ProFlex PCR System easy and efficient to share in busy labs.

Watch the "What if is now" ProFlex video ›

Quickly, easily swap out blocks to meet your PCR needs

The ProFlex PCR System has 5 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well block. This block allows up to three experiments to be run simultaneously, completely independently of each other and without interference. Dual 96-well and dual 384-well blocks are available for your high-throughput sequencing needs, and a dual flat block is available to support Applied Biosystems OpenArray plate technology for genotyping analysis on the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System.

3 x 32-well block for running up to three independent experiments simultaneously.
96-well block for standard PCR applications.
Dual 96-well block for high-throughput sequencing.
Dual 384-well block for higher-throughput sequencing.
Dual flat block supports OpenArray plate technology for genotyping analysis on the QuantStudio 12K Flex Real-Time PCR System.

Boost productivity with secure remote access to your ProFlex thermal cycler

The ProFlex thermal cycler is a cloud-enabled instrument, allowing you secure private access with a Thermo Fisher Connect account. Design and share protocols and monitor your runs from anywhere, anytime, with any mobile device or desktop computer. Setup is easy, and your account comes with 10 GB of free storage.

View quick setup

Store Store

Design protocols from anywhere, store in the cloud.

Develop protocols on any desktop computer or mobile device and store them in your secure personal cloud account. When you are ready to prepare your run, simply access your cloud account from your ProFlex thermal cycler.

Store Monitor

Remote monitoring of runs in real-time.

Download our Instrument Connect remote monitoring app to see availability and run progress, catch issues while they are in progress, and more on your ProFlex thermal cycler from anywhere, anytime.

Store Share

Discretionary access to project folders and protocols.

Share your protocols or other files with other Thermo Fisher Connect users on campus and around the world. It’s easy, convenient, and protected by the highest levels of privacy standards.

Learn more about Thermo Fisher Connect ›

For peace of mind, the Thermo Fisher Cloud has high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services™ (AWS) to offer a robust platform.

Precise PCR optimization with VeriFlex technology

VeriFlex Blocks provide a better-than-gradient approach to PCR optimization. With three separate Peltier blocks, you have precise control over three temperature zones.

  • Enables highly accurate determination of optimal annealing temperature
  • Helps eliminate guesswork often associated with standard gradient cyclers
  • Helps save time by running up to three reactions with different annealing temperatures in the same PCR run
  • Use your thermal cycler as an incubator; take advantage of precision temperature control

Applied Biosystems PCR plastics

Applied Biosystems plastic consumables offer excellent PCR and qPCR performance in formats developed to meet your experimental needs. All of our plastic consumables are validated with Applied Biosystems instruments for optimal fit and performance. Consistent, precise results.


Applied Biosystems Thermal Cycler, Plastics and Services Brochure

PCR and cDNA Synthesis Support Center 
Tips and troubleshooting help

School of Molecular Biology, PCR Education
Technical content for new and experienced molecular biologists

Thermal Cycler Software and Firmware Files
Download the latest updates for your instrument

Interested in private label thermal cyclers?
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We provide award-winning service options for all Applied Biosystems thermal cyclers.

Service plans

  • Rapid Exchange minimizes interruptions from unplanned instrument downtime
  • Global Repair Center provides a cost-efficient way to maximize uptime and achieve superior performance

Additional services available

Instrument qualification service: Have one of our engineers perform temperature qualifications at your site. You may also contact Instrument Services to purchase a temperature verification tool and perform the qualification yourself.