Thermo Scientific™ MSIA™ products are for laboratory and life science research only.

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Featured Resource

What is MSIA?

Mass Spectrometric Immunoassay (MSIA) microcolumns are coated with affinity ligands giving you the power to capture even low abundant analytes from the most complex samples. MSIA can be used on low- and high- throughput formats and help to enable simple, reproducible and precise affinity capture—suitable for your bioanalysis workflow.


Application notes


Brochures


Technical manuals


Protocol examples


Webinars

Enzyme Acceleration-based Immunoassays

Webinar: Enzyme Acceleration-based Immunoassays Using Thermo Scientific MSIA Affinity Capture Technology.

Duration: 58 minutes
Download


MSIA Workflow for Insulin Purification and MS Analysis

This webinar examines the use of the MSIA Insulin workflow for the purification and MS analysis of insulin analogs for clinical research applications.

Duration: 60 minutes 
Download

Keeping it clean using MSIA Microcolumns

Keeping it clean using MSIA Microcolumns with mass spectrometry to analyse therapeutic peptides.

Duration: 57 minutes
Download


MSIA Workflow for Therapeutic Antibody Analysis

Watch and learn from a recorded webinar on July 1, 2014 that shows how to use a MSIA for the purification and MS analysis of therapeutic antibodies.

Duration: 60 minutes
Download

Overcoming Challenges in ADC Bioanalysis

This webinar features MSIA products and examines the innovation of biotherapeutics and the challenge presented in both analytics and bioanalysis.

Duration: 50 minutes
Download


Videos

Improving performance for Tecan
 

Iimproving performance of clinical research through a straightforward, automated workflow utilizing Thermo Scientific MSIA technology for the Tecan Freedom EVO Platform. 

Explore our MSIA technology
 

Learn how to streamline mass spectrometry-based quantitative proteomics with MSIA pipette tips! A simple way to enrich and concentrate target proteins down to the picogram amount.

Accelerate bioanalysis with MSIA workflows

Watch how researchers are using MSIA workflows for fast and easy affinity purification and downstream MS detection of target proteins, insulin variants, and therapeutic antibodies.


Publications

Bieber, A. L., Tubbs, K. A., and Nelson, R. W., Metal ligand affinity pipettes and bioreactive alkaline phosphatase probes: Tools for characterization of phosphorylated proteins and peptides, Mol. Cell. Proteomics. 3, 266-272 (2004).

Dogruel, D., Williams, P., and Nelson, R. W., Rapid tryptic mapping using enzymatically active mass spectrometer probe tips, Anal. Chem., 67, 4343 (1995).

Flynn, C. R., Komalavilas, P., Tessier, D., Thresher, J., Niederkofler, E. E., Dreiza, C. M., Nelson, R. W., Panitch, A., Joshi, L., and Brophy, C. M., Transduction of biologically active motifs of the small heat shock-related protein HSP20 leads to relaxation of vascular smooth muscle, FASEB J. 17 , 1358-1360 (2003).

Kiernan, U. A., Addobbati, R., Nedelkov, D., and Nelson, R. W., Quantitative multiplexed C-reactive protein mass spectrometric immunoassay, J. Proteome Res., 5, 1682-7 (2006).

Kiernan, U. A., Black, J. A., Williams, P., and Nelson, R. W., High-throughput analysis of hemoglobin from neonates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Clin. Chem. 48, 947 (2002).

Kiernan, U. A., Nedelkov, D., and Nelson, R. W., Multiplexed mass spectrometric immunoassay in biomarker research: a novel approach to the determination of a myocardial infarct, J. Proteome Res., 5, 2928-34 (2006).

Kiernan, U. A., Nedelkov, D., Niederkofler, E. E., Tubbs, K. A., and Nelson, R. W., High-throughput affinity mass spectrometry, Methods Mol. Biol., 328, 141-50 (2006).

Kiernan, U. A., Nedelkov, D., Tubbs, A. T., Niederkofler, E. E., and Nelson, R. W., Proteomic characterization of novel serum amyloid P component variants from human plasma and urine, Proteomics 4, 1825-1829 (2004).

Kiernan, U. A., Tubbs, K. A., Gruber, K., Nedelkov, D., Niederkofler, E. E., Williams, P. and Nelson, R. W., High-throughput protein characterization using mass spectrometric immunoassay, Anal. Biochem., 301, 49 (2002).

Kiernan, U. A., Tubbs, K. A., Nedelkov, D., Niederkofler, E. E., and Nelson, R. W., Detection of novel truncated forms of human serum amyloid A protein in human plasma, FEBS Lett. 537, 166-170 (2003).

Kiernan, U. A., Tubbs, K. A., Nedelkov, D., Niederkofler, E. E., and Nelson, R. W., Comparative phenotypic analyses of human plasma and urinary retinol binding protein using mass spectrometric immunoassay, Biochem. Biophys. Res. Commun. 297, 401 (2002).

Kiernan, U. A., Tubbs, K. A., Nedelkov, D., Niederkofler, E. E., McConnel, E., and Nelson, R. W., Comparative urine protein phenotyping using mass spectrometric immunoassay, J. Proteome Res. 2191-197 (2003).

Kiernan, U. K., Nedelkov, D., Tubbs, A. T., Niederkofler, E. E., and Nelson, R. W., Selected expression profiling of full-length proteins and their variants in human plasma, Clin. Proteomics 1, 7-16 (2004).

Kiernan, U.A., Hernandez, L., Niederkofler, E.E., Tubbs, K.A., and Nelson, R.W., MS-based phenotypic characterization of a human blood protein from urinary waste products, Proteomics Clin. Appl., 2, 1019-1024 (2008).

Kiernan, U.A., Phillips, D.A., Trenchevska, O., and Nedelkov, D., Quantitative mass spectrometry evaluation of human retinol binding protein 4 and related variants, PLoS One. 6(3): e17282 (2011).

Krone, J. R., Nelson, R. W., Dogruel, D., Williams, P., and Granzow, R., BIA/MS: interfacing biomolecular interaction analysis with mass spectrometry, Anal. Biochem., 244, 124 (1997).

Lee, H. J., Nedelkov, D., and Corn, R. M., Surface plasmon resonance imaging measurements of antibody arrays for the multiplexed detection of low molecular weight protein biomarkers, Proteomics, 5, 5002-7 (2005).

Lewis, J. K., Krone, J.R., and Nelson, R. W., Mass spectrometric methods for evaluating point mutations, Biotechniques, 1, 102 (1998).

Lopez, M.F., Rezai, T., Sarracion, D.A., Prakash, A., Krastins, B., Athanas, M., Singh, R.J., Barnidge, D.R., Oran, P., Borges, C., and Nelson, R.W., Selected Reaction Monitoring - Mass Spectrometric Immunoassay Responsive to Parathyroid Hormone and Related Variants, Clin. Chem., 281-290 (2010).

Lukas, R. J., Tubbs, K. A., Krivoshein, A. V., Bieber, A. L., and Nelson, R. W., Mass Spectrometry of nicotinic acetylcholine receptors and associated proteins as models for complex transmembrane proteins, Anal. Biochem., 301, 175 (2002).

Nedelkov, D., Development of surface plasmon resonance mass spectrometry array platform, Anal. Chem., 79, 5987-5990 (2007).

Nedelkov, D., Mass spectrometry-based immunoassays for the next phase of clinical applications, Expert Rev., Proteomics, 3, 631-640 (2006).

Nedelkov, D. and Nelson, R. W., Biomolecular Interaction Analysis Mass Spectrometry, in: Silberring, J. and Ekman, R. (eds), Mass spectrometry and hyphenated techniques in neuropeptide research, Wiley, London, pp. 215-233 (2002).

Nedelkov, D. and Nelson, R. W., Delineation of in-vivo assesmbled multi-protein complexes via Biomolecular Interaction Analysis Mass Spectrometry, Proteomics 1, 1441 (2001).

Nedelkov, D. and Nelson, R. W., Analysis of native proteins from biological fluids by Biomolecular Interaction Analysis Mass Spectrometry: Exploring the limit of detection, identification of non-specific binding and detection of multi-protein complexes, Biosens. Bioelec. 16,1071 (2001).

Nedelkov, D. and Nelson, R. W., Biomolecular Interaction Analysis Mass Spectrometry, Bull. Chem. Technol. Macedonia, 20, 19 (2001).

Nedelkov, D. and Nelson, R. W., Analysis of human urine protein biomarkers via Biomolecular Interaction Analysis Mass Spectrometry, Am. J. Kid. Dis., 38, 481 (2001).

Nedelkov, D. and Nelson, R. W., Biomolecular Interaction Analysis Mass Spectrometry: A multiplexed proteomics approach, BioPharm., July 2001, 28.

Nedelkov, D., and Nelson, R. W., Surface plasmon resonance mass spectrometry for protein analysis, Methods Mol. Biol, 328, 131-9 (2006).

Nedelkov, D., and Nelson, R. W., Biomolecular Interaction Analysis Mass Spectrometry (BIA/MS), in: Cellis J. E. (Ed.), Cell Biology: A laboratory handbook, Third Ed., Elsevier., in press (2004).

Nedelkov, D., and Nelson, R. W., Detection of staphylococal enterotoxin B via biomolecular interaction analysis mass spectrometry, Appl. Environ. Microbiol. 69, 5212-5215 (2003).

Nedelkov, D., and Nelson, R. W., Surface plasmon resonance mass spectrometry: recent progress and outlooks, Trends Biotecnol. 21, 301-305 (2003).

 

Nedelkov, D., and Nelson, R. W., Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): a step toward the design of SPR/MS arrays, J. Mol. Recogn. 16, 15-19 (2003).

Nedelkov, D., and Nelson, R. W., Delineating protein-protein interactions via biomolecular interaction analysis-mass spectrometry, J. Mol. Recogn. 16, 9-14 (2003).

Nedelkov, D., and Nelson, R. W., Exploring the limit of detection in biomolecular interaction analysis mass spectrometry (BIA/MS): detection of attomole amounts of native proteins present in complex biological mixtures, Anal. Chim. Acta., 423, 1 (2000).

Nedelkov, D., and Nelson, R. W., Practical considerations in BIA/MS: optimizing the biosensor-mass spectrometry interface, J. Mol. Recognit., 13, 140 (2000).

Nedelkov, D., Kiernan, U. A., Niederkofler, E. E., Tubbs, K. A., and Nelson, R. W., Population proteomics: the concept, attributes, and potential for cancer biomarker research, Mol. Cell Proteomics, 5, 1811-8 (2006).

Nedelkov, D., Kiernan, U. A., Niederkofler, E. E., Tubbs, K. A., and Nelson, R. W., Investigating diversity in human plasma proteins, PNAS 102, 10852-857 (2005).

Nedelkov, D., Nelson, R. W., Kiernan, U. A., Niederkofler, E. E., and Tubbs., K. A., Detection of bound and free IGF-1 and IGF-2 in human plasma via biomolecular interaction analysis mass spectrometry, FEBS Lett. 536, 130-134 (2003).

Nedelkov, D., Phillips, D. A., Tubbs, K. A., and Nelson, R. W., Investigation of human protein variants and their frequency in the general population, Mol. Cell Proteomics, 6, 1183-1187 (2007).

Nedelkov, D., Rasooly, A., and Nelson, R. W., Multi-toxin biosensor/mass spectrometry analysis: a new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food, Intl. J. Food Micr., 60, 1 (2000).

Nedelkov, D., Tubbs, K. A., and Nelson, R. W., Surface plasmon resonance-enabled mass spectrometry arrays, Electrophoresis, 27, 3671-5 (2006).

Nedelkov, D., Tubbs, K. A., and Nelson, R. W., Design of buffer exchange surfaces and sensor chips for Biosensor Chip Mass Spectrometry, Proteomics 2, 441, 2002.

Nedelkov, D., Tubbs, K. A., Niederkofler, E. E., Kiernan, U. A., and Nelson, R. W., High-throughput comprehensive analysis of human plasma proteins: a step toward population proteomics, Anal. Chem. 76, 1733-1737 (2004).

Nedelkov, Dobrin, Population proteomics: addressing protein diversity in humans, Expert Rev. Proteomics 2, 315-324 (2005).

Nelson, R. W., The use of bioreactive probes in protein characterization, Mass Spectrom. Rev., 16, 353 (1997).

Nelson, R. W., and Krone, J. R., Advances in surface plasmon resonance biomolecular interaction analysis mass spectrometry (BIA/MS), J. Mol. Recognit., 12, 77 (1999).

Nelson, R. W., Dogruel, D., Krone, J. R., and Williams, P., Peptide characterization using bioreactive mass spectrometer probe tips, Rapid Commun. Mass Spectrom., 9, 1380 (1995).

Nelson, R. W., Jarvik, J. W., Taillon, B. E., and Tubbs, K. A., BIA/MS of epitope-tagged peptides directly from E. coli lysate: multiplex detection and protein identification at low-femtomole to subfemtomole levels, Anal. Chem., 71, 2858 (1999).

Nelson, R. W., Krone, J. R., and Jansson, O., Surface plasmon resonance biomolecular interaction analysis mass spectrometry 2. Fiber optic-based analysis, Anal. Chem., 69, 4369 (1997).

Nelson, R. W., Krone, J. R., and Jansson, O., Surface plasmon resonance biomolecular interaction analysis mass spectrometry. 1. Chip-based analysis, Anal. Chem., 69, 4363 (1997).

Nelson, R. W., Krone, J. R., Bieber, A. L., and Williams, P., Mass-Spectrometric Immunoassay, Anal. Chem., 67, 1153 (1995).

Nelson, R. W., Krone, J. R., Dogruel, D., Tubbs, K. A., Granzow, R., and Jansson, O., Interfacing biomolecular interaction analysis with mass spectrometry and the use of bioreactive mass spectrometer probe tips in protein characterization, Techniques in protein chemistry VIII , Marshak, D., Ed., Academic Press, San Diego, pp. 493 (1997).

Nelson, R. W., Krone, J. R., Tubbs, K. A., and Dogruel, D., Mass spectrometric methods for biomolecular characterization, in New methods for the study of biomolecular complexes, Ens, W., Standing, K. G., and Chernushevich, I. V., Eds., Kluwer, Dordrecht, pp. 225 (1998).

Nelson, R. W., Nedelkov, D., and Tubbs, K. A., Biosensor chip mass spectrometry: a chip-based proteomics approach, Electrophoresis, 21, 1155 (2000).

Nelson, R. W., Nedelkov, D., and Tubbs, K. A., Biomolecular Interaction Analysis Mass Spectometry. BIA/MS can detect and characterize protiens in complex biological fluids at the low- to subfemtomole level, Anal. Chem., 72, 404A (2000).

Nelson, R. W., Nedelkov, D., Tubbs, K., and Kiernan, U. A., Quantitative Mass Spectrometric Immunoassay of Insulin Like Growth Factor 1, J. Proteome Res. (4), 851–855 (2004).

Niederkofler, E. E., Tubbs, K. A., Gruber. K, Nedelkov, D., Kiernan, A. U., Williams, P. and Nelson, R. W., Determination of beta-2-microglobulin levels in plasma using a high-throughput mass spectrometric immunoassay system, Anal. Chem., 73, 3294 (2001).

Niederkofler, E. E., Tubbs, K. A., Kiernan, U. A., Nedelkov, D., and Nelson, R. W., Novel mass spectrometric immunoassays for the rapid structural characterization of plasma apolipoproteins, J. Lipid Res. 44, 630-639 (2003).

Niederkofler, E.E., Kiernan, U.A., O'Rear, J., Menon, S., Saghir, S., Protter, A.A., Nelson, R.W., and Schellenberger, U., Detection of Endogenous B-Type Natriuretic Peptide at Very Low Concentrations in Patients with Heart Failure, Circulation: Heart Failure , 1, 258-264 (2008).

Trenchevska, O., and Nedelkov, D., Targeted Quantitative Mass Spectrometric Immunoassay for Human Protein Variants, Proteome Sci. 9:19 (2011).

Trenchevska, O., Kamcheva, E., and Nedelkov, D., Mass spectrometric immunoassay for quantitative determination of protein biomarker isoforms, J. Proteome Res. 9, 5969 (2010).

Tubbs, K. A., Kiernan, U. A., Niederkofler, E. E., Nedelkov, D., Bieber, A. L., and Nelson, R. W., Development of recombinant-based mass spectrometric immunoassay with application to resistin expression profiling, Anal. Chem., 78, 3271-6 (2006).

Tubbs, K. A., Kiernan, U. A., Niederkofler, E. E., Nedelkov, D., Bieber, A. L., and Nelson, R. W., High-throughput MS-based protein phenotyping: Application to haptoglobin, Proteomics, 5, 5002-7 (2005).

Tubbs, K. A., Nedelkov, D. and Nelson, R. W., Detection and quantitation of b-2-microglobulin using mass spectrometric immunoassay, Anal. Biochem., 289, 26 (2001).

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