Chromogenic Western Blot Detection
Unlike chemiluminscent or fluorescent blotting applications, chromogenic substrates do not require special equipment for visualization of the assay results. Similar to developing film, the blot is incubated in substate until the desired amount of development. Unlike chemiluminescent blotting assays, the colored precipitate cannot be easily be stripped off for reprobing.
Which chromogenic detection kit is right for you?
|1-Step Chloronaphthol||Metal-enhanced DAB Substrate Kit||CN/DAB Substrate Kit||1-Step Ultra TMB Blotting Solution|
|Features||Stable at 4°C for 1 year||Working solution is stable for over six hours at RT||Stable at 4°C for 1 year||Stable at 4°C for 1 year|
|Sensitivity||5 ng||17 pg||500 pg||20 pg|
|Order now||Order now||Order now||Order now|
Additional chromogenic kits
WesternBreeze® Chromogenic Kits
WesternBreeze® Chromogenic Kits yield high-sensitivity results with extremely low background without the need for any additional equipment—just watch the signal develop over a short period of time. The kit uses the chromogenic phosphatase substrate BCIP (5-bromo-4-chloro-3-indolyl phosphate) and electron-transfer agent NBT (nitro blue tetrazolium) to produce a dark blue precipitate at the precise site of enzymatic activity on the blot.
The WesternBreeze® kit has been optimized to provide picogram sensitivity. Each kit includes ready-to-use or easy-to-dilute blockers, primary antibody diluent, wash solution, conjugated secondary antibody solution, substrate, and two convenient incubation trays.
Pierce™ NBT and BCIP Substrates are ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce™ 1-Step NBT/BCIP solutions are available with and without a levamisole suppressor of endogenous phosphatase activity.
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For Research Use Only. Not for use in diagnostic procedures.