One of the key steps in the western blot workflow is the transfer of proteins from the polyacrylamide gel after electrophoresis to the nitrocellulose or polyvinylidene difluoride (PVDF) membrane so that specific proteins can be detected using immunodetection techniques.

Methods for achieving this protein transfer include capillary transfer or electrophoretic transfer. We have developed electrophoretic transfer systems using wet, semi-dry, and dry methods outlined below. Use the table below to select the appropriate transfer method for your western blotting needs.

Which western transfer system is right for you?

  Traditional wet transfer Semi-dry transfer Dry transfer
  XCell II™ Blot Module
Mini Blot Module
Novex® Semi-Dry Blotter Pierce® Power Blotter iBlot® 2 Gel Transfer Device
Transfer time 60–120 min 60 min 30–60 min 5–10 min 7 min
Capacity of device 1–2 mini gels 1 mini gel (per module) or 2 mini gels (two modules per tank) 4 mini-gels or
2 midi-gels
4 mini gels or
2 midi gels
2 mini gels or
1 midi gel
Blotting area 9 x 9 cm 9 x 9 cm 21 x 21 cm 21 x 22.5 cm 8.5 x 13.5 cm
Requires transfer buffer Yes (1,000 mL) Yes, (200–400 mL per module) Yes (500 mL) Yes (50 mL per mini gel or 100 mL per midi gel) No
Power supply External External External Internal Internal
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