iBlot® 2 Dry Blotting System  

Find the quick reference card, instruction manual, and FAQs for the iBlot® 2 Gel Transfer Device, and be sure to register your device so that you can receive the latest product information including firmware updates.

If you are looking for technical resources for the original iBlot® device, see Reagents and Resources for the Original iBlot® Gel Transfer Device.

Registration and firmware

Register your device to access the newest versions of firmware for the iBlot® 2 Gel Transfer Device as they become available.

Note for IE users: (1) right-click the above “Download” link; (2) click “Save Target As”; (3) Change file name to “image.ib2”; (4) Select file type as “All Files”; (5) click “Save”

iBlot2 firmware upgrade instructions

Frequently Asked Questions

No. Do not use iBlot® Transfer Stacks in the iBlot® 2 Gel Transfer Device, and do not mix components between iBlot® Transfer Stacks and iBlot® 2 Transfer Stacks.

Yes, all original iBlot® stacks are still available for purchase. You can find them in the original iBlot® Gel Transfer Device Reagents and Resources.

It is currently 9 months, and the expiration date is printed on the box.

No. The results may be inferior after the expiration date.

Yes. However, minor optimizations in the transfer protocol (i.e., steps, voltage, time) may be needed for some proteins.

Yes. The device allows programming custom methods.

Yes. The device is designed to do so with no impact on performance.

No. They are disposable and should only be used once.

The membranes are compatible with all commonly used detection methods such as staining, immunodetection, fluorescence, etc.

It is possible but the results may be inferior.

Yes. The PVDF membrane is pre-activated and ready for use without having to perform any pre-treatment with alcohol.

No. Maintain the membrane size identical to the transfer stacks. This helps ensure that there is no direct contact between the top and bottom transfer stacks.

No. Do not trim the iBlot® 2 Transfer Stacks to fit your gel size.