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Western Blotting Information
Learn about methods and technologies for western blot detection and imaging. Understand the reagents and instruments used for the efficient membrane transfer and detection of proteins, including wash and blocking buffers, secondary antibody conjugates, and chemiluminescent, colorimetric or fluorescent detection substrates.
Western blotting features
This 84-page handbook provides a deep dive about the last step in the western blot workflow—protein detection. With a variety of detection techniques to choose from (chemiluminescence, fluorescence or chromogenic), you can select a technology to match your experimental requirements and the instruments you have available.
Videos that demonstrate each step in the western blot workflow, including SDS-PAGE, transfer, blocking, probing with antibodies, chemiluminescent detection, and stripping the blot.
Western blotting learning resources
For Research Use Only. Not for use in diagnostic procedures.