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Finding the right protein gel electrophoresis system is critical to getting the consistent and accurate results you need for your research. Reagents and buffers are important components of the protein electrophoresis system. We offer a range of Invitrogen™ Novex™ reagents and buffers for gel casting and sample preparation and for running, staining and drying gels.

SureCast Gel Handcast Reagents

Gel electrophoresis buffers and reagents

Below you will find our recommendations for sample preparation, running buffers and other reagents for each type of protein gel electrophoresis systems that we offer, including native gel electrophoresis, zymography, and isoelectric focusing.


Native electrophoresis recommended buffers & reagents

Gel type Sample preparation reagents Running buffer Transfer buffer Buffer kit*
Novex WedgeWell Tris-Glycine gelsl  
NativePAGE™ Bis-Tris Gel
*NativePAGE Sample Prep Kit—contains NativePAGE Sample Buffer (4X), 5% G-250 Sample Additive, 10% DDM, and 5% digitonin; NativePAGE Running Buffer Kit—contains NativePAGE Running Buffer (20X) and NativePAGE Cathode Buffer Additive (20X)

Zymography gel recommended buffers & reagents


IEF gel recommended buffers

Gel type Sample buffer Running buffer Buffer kit*
Novex IEF Gel

*Novex pH 3-10 IEF Buffer Kit contains Novex IEF Anode Buffer (50X), Novex IEF Cathode Buffer pH 3-10 (10X), and Novex IEF Sample Buffer pH 3-10 (2X); Novex pH 3-7 IEF Buffer Kit contains Novex IEF Anode Buffer (50X), Novex IEF Cathode Buffer pH 3-7 (10X), and Novex IEF Sample Buffer pH 3-7 (2X)


Gel staining and drying solutions and reagents

Below are selected products for gel staining and drying. Learn more about our Protein Gel Staining and Imaging products and about our Protein Gel Staining and Drying Accesories.

Novex gel cassette video