NuPAGE Tris-Acetate Gels

Invitrogen NuPAGE Tris-Acetate Gels are high-performance gels that have been optimized for separating proteins of large molecular weight.

Performance dataInteractive selection tool

Available NuPAGE Tris-Acetate Gels
Migration charts

NuPAGE Tris-Acetate Gels are designed to give optimal separation of large molecular weight proteins during gel electrophoresis. In comparison to traditional tris-glycine SDS-PAGE gels, NuPAGE Tris-Acetate Gels have an environment of pH 8.1, which minimizes protein modifications and results in sharper bands.

 Features of NuPAGE Tris-Acetate Gels include:

  • High resolution—gels offer optimal separation of high molecular weight proteins
  • Better protein integrity—sample preparation process has been optimized to help preserve your proteins
  • Longer shelf life—gels can be stored for at least 8 months

NuPAGE Tris-Acetate Gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using LDS sample buffer and tris-acetate SDS running buffer. For native proteins, traditional tris-glycine native sample buffer and a tris-glycine native running buffer should be used.

The NuPAGE Tris-Acetate Gels use a discontinuous buffer system involving three ions:

  • Acetate (–) is from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are tris (+) and acetate (–) (pH 7.0).
  • Tricine (–) is from the running buffer and serves as the trailing ion. The running buffer ions are tris (+), tricine (–) and dodecylsulfate (pH 8.3).
  • Tris (+) is the common ion present in the gel buffer and running buffer. The tris-acetate system also operates at a significantly lower operating pH of 8.1 during electrophoresis. 
Available gel sizes Mini: 8 cm x 8 cm (1 or 1.5 mm thick) 
Midi: 8 cm x 13 cm (1 mm thick)
Storage conditions Room temperature
Shelf life 8 months
Recommended sample buffer LDS sample buffer or native tris-glycine sample buffer
Recommended running buffers Tris-acetate running buffer
Gel chemistry Tris-acetate
Available polyacrylamide concentrations 7%, 3–8%
Separation range (denaturing) 30–500 kDa
For use with (equipment) mini gels Mini Gel Tank or XCell SureLock Mini-Cell
For use with (equipment) midi gels Bio-Rad Criterion (with adapters only) or Invitrogen XCell4 SureLock Midi-Cell
Mode of separation Molecular weight

Currently using Tris-glycine or Tricine gels? Use the NuPAGE Gel conversion guide below to find the appropriate NuPAGE gel and corresponding optimized buffer.

NuPAGE Gel conversion guide

If you are using: Then we recommend NuPAGE gel: Buffer system
Tris-Glycine gel
4% NuPAGE 3-8% Tris-Acetate Tris-Acetate
6% NuPAGE 3-8% Tris-Acetate Tris-Acetate
10% NuPAGE 8% Bis-Tris MOPS
NuPAGE 10% Bis-Tris MOPS
12% NuPAGE 10% Bis-Tris MOPS
14% NuPAGE 12% Bis-Tris MOPS
16% NuPAGE 12% Bis-Tris MES
18% NuPAGE 12% Bis-Tris MES
4-12% NuPAGE 3-8% Tris-Acetate Tris-Acetate
NuPAGE 4-12% Bis-Tris MOPS
4-20% NuPAGE 4-12% Bis-Tris MES
8-16% NuPAGE 4-12% Bis-Tris MOPS
10-20% NuPAGE 12% Bis-Tris MOPS
Tricine gel
10% NuPAGE 8% Bis-Tris MES
NuPAGE 10% Bis-Tris MES
16% NuPAGE 4-12% Bis-Tris MES
10-20% NuPAGE 4-12% Bis-Tris MES
Visistat reference component