E-PAGE High-Throughput gel system

The Invitrogen E-PAGE High-Throughput (HTP) Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis.

E-Editor 2.0 SoftwareAvailable E-PAGE Gels

Migration charts

The E-PAGE High-Throughput (HTP) Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis. Invitrogen E-PAGE Gels are self-contained, precast gels that include a gel matrix and electrodes packaged inside a disposable cassette. E-PAGE Gels are available in 48-well or 96-well formats.

Features of the E-PAGE High-Throughput Precast Gel System include:

  • Ease-of-use—Quick setup and fast separations (23 minutes) for 1D protein analysis
  • Fast loading—Compatible with multichannel pipettors and robotic loading
  • Efficient western blotting and staining—Optimized protocols and reagents

Protein separation on an E-PAGE Gel requires an Invitrogen Mother E-Base Device, a compact and automated platform designed for medium- and high-throughput electrophoresis.

Invitrogen E-Base integrated devices combine the base and the power source in one device. Up to 3 Invitrogen Daughter E-Base Devices may be connected to one Mother E-Base Device to permit multiple gels to be run simultaneously using identical parameters. E-Base integrated devices are compatible with the standard plate size of the Society for Biomolecules Screening (SBS) and can be conveniently mounted on liquid handling robot decks.

E-PAGE Gels

We offer two kinds of E-PAGE Gels:

  • Invitrogen E-PAGE  8% Protein Gels, 48-well—Each gel contains 48 sample lanes and 4 marker lanes with a 3.2-cm separation length, and can be loaded with a 12- or 8-channel pipettor or 4- or 8-tip robotic system.
  • Invitrogen E-PAGE 6% Protein Gels, 96-well—Each gel contains 96 sample lanes and 8 marker lanes in a patented staggered-well format with a 1.6-cm run length; well openings are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid handling robotic device.

Both E-PAGE 48-well and 96-well cassettes are conveniently opened with a butterfly opener to remove the gel for downstream staining or blotting. In addition, each cassette is labeled with a unique barcode to facilitate identification of the gel using commercial barcode readers.

Available gel sizes 48-well (3.7 mm thick)
96-well (3.7 mm thick)
Storage conditions Room temperature 
Shelf life 6–9 months
Available polyacrylamide concentrations 6% (96 wells), 8% (48 wells) 
Separation range 10–200 kDa
For use with (equipment) mini gels E-Base integrated devices
Mode of separation Molecular weight
  E-PAGE 48-well 8% gels E-PAGE 96-well 6% gels
Cassette size 13.5 (l) x 10.8 (h) x 0.67 cm (w) 13.5 (l) x 10.8 (h) x 0.67 cm (w)
Gel thickness 3.7 mm 3.7 mm
Gel volume 50 mL 50 mL
Well depth 3 mm 3 mm
Number of wells 48 + 4 markers 96 + 8 markers
Well format 2 x 26 Staggered 8 x 13
Well opening 3.6 mm x 2.2 mm 3.8 mm x 1.8 mm
Running distance 32 mm 16 mm
Run time 23 min 14 min
Space between well centers 4.5 mm 9 mm
Sample volume 15 µL 20 µl
Maximum protein load 20 µg 20 µg

 

 

Migration pattern of Invitrogen E-PAGE MagicMark Unstained Protein Standard.