Protein purification products and technologies

Find the best tools for your workflow from our broad menu of resins and formats for the purification of recombinant proteins and antibodies. Create a customized purification solution by covalent attachment of a ligand to one of our activated supports. Alternatively, choose one of our easy-to-use and efficient agarose- or magnetic bead–based kits for immunoprecipitation (IP) and co-IP applications. In addition, we’ve developed a range of accessory products for all aspects of purification, including disposable columns and binding and elution buffers.

  • Broad product selection—affinity supports for the purification and enrichment of antibodies, recombinant proteins, and biotinylated proteins, as well as activated supports for custom protein immobilization
  • More formats—magnetic beads, loose resin, FPLC cartridges, and 96-well filter plates enable protein purification from screening and small-scale phases to process-scale purification
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing that is similar to or better than our leading competitors

Featured protein purification categories

We offer multiple formats and packs sizes of Protein A, G, A/G, and Protein L immobilized resins that have specific affinity to immunoglobulins, allowing purification of all antibodies of a particular target class regardless of antigen specificity.

Protein enrichment strategies involve the selective isolation of protein subclasses sharing a common posttranslational modification (PTM), such as phosphorylation and glycosylation, typically via affinity ligands such as ion-metal affinity chromatography (IMAC) or immobilized lectins.

We offer a broad range affinity purification products designed for the purification of recombinant proteins—specifically cobalt and nickel IMAC resins for His-tagged proteins and immobilized glutathione resins for GST-tagged proteins. Small-scale resins are also available for c-myc and HA-tagged proteins.

We offer kits in a range of formats for various immunoprecipitation (IP) and co-immunoprecipitation (co-IP) strategies, as well as products for capturing protein-nucleic acid interactions (ChIP and RIP) and solutions for enriching recombinant or modified proteins (pull-down assays).

The strong association between avidin and biotin has been successfully employed in various affinity purification schemes. We offer a variety of immobilized biotin binding supports, including avidin, streptavidin, NeutrAvidin®, CaptAvidin®, and monomeric avidin resins.

Magnetic beads are optimized for protein enrichment from small volume samples with low protein concentrations, such as immunoprecipitation (IP), co-IP, and pull-down assays. Magnetic beads are ideal for assay automation, and are recommended for small-scale and/or screening applications.

We’ve developed activated supports for the immobilization of proteins and other molecules for target-specific affinity purification. The functional groups on the resins utilize a range of coupling mechanism for immobilizing amine-, sulfhydryl-, carbonyl-, and carboxyl-based targets.

For your convenience, we offer a wide range of Thermo Scientific™ disposable plastic columns (spin- and gravity-based) for small-scale purification needs, BupH Dry-Blend Buffer Packs, and Pierce® 10X and 20X Concentrated Buffers. 

Related Products