Mutations in mitochondrial DNA have been linked to diabetes and aging, as well as the development of certain cancers. Mitochondrial DNA resequencing is therefore a useful tool for researchers looking to better understand these diseases.

Step-by-Step Guide to Mitochondrial Sequencing Products

DNA Purification kit

DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.

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BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions.

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DNA strands

BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. PCR Clean up and sequencing reaction are perform in a single step.

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BigDye® Direct cycle sequencing kits

Sequencing Reaction Cleanup

After the sequencing reaction, it is important to remove unincorporated dye terminators and salts that may compete for capillary electrophoretic injection. Unincorporated terminators can co-migrate with the sequencing template, resulting in basecalling er

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Which Sample Cleanup Product Is Right for You?

Cleanup Method Description & Application Product
Reagent-Based Sequencing Template Cleanup Scavenges all unincorporated BigDye® terminators. Stabilizes samples before analysis. Best for long and short fragment recovery.
Capillary Electrophoresis

During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.

Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You?

Number of Capillaries 96 48 24 8 16 4 1
Compatible Applications:
(S) Supported; (A) AB Demonstrated; (C) Customer Demonstrated; (N) Not Supported
mtDNA Sequencing S S S S S S S

Analyze the mitochondrial sequence.

Which Data Analysis Software Is Right for You?

Highlighted products for Mitochondrial Sequencing:

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