Comprehensive gene coverage with the least amount of DNA

Ion AmpliSeq™ technology delivers simple and fast library construction for affordable targeted sequencing of specific genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq technology requires as little as 1 ng of input DNA to target sets of genes, making sequencing of FFPE samples routine on Ion PGM™ or Ion S5™ systems.

Alternative targeted sequencing and enrichment methods are lengthy and complex, and they require large amounts of DNA, making them impractical for sequencing DNA from the formalin-fixed, paraffin-embedded (FFPE) tissues that are commonly used in translational and clinical research. Using as little as 1 ng of FFPE DNA and one pool of thousands of primer pairs, variants can be identified in a single day using the Ion AmpliSeq custom workflow. By contrast, the same project using alternative workflows can require up to 100 times as much DNA, 8 times the pools, and twice the time.

Watch how it works ›


  • 15 minutes of hands-on time for automated library preparation


  • Up to 24,000 targets per tube
  • One to hundreds of genes
  • Up to 384 barcodes for multiplexing


  • As little as 24 hours from FFPE sample to results
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Choose from a wide variety of Ion AmpliSeq targeted sequencing panels to transform your research:

The convenient predesigned Ion AmpliSeq™ ready-to-use panels allow researchers to focus on data generation and analysis, not on the labor-intensive primer design and target selection steps.

Choose from over 20,000 genes to survey gene expression fold changes starting with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA.

For maximum flexibility, simply select a subset of targets and enter your specific genomic content online using the Ion AmpliSeq™ Designer tool, and we will design and create customized Ion AmpliSeq panels just for you. Choose from a number of reference genomes or upload your own reference sequence.

The Ion AmpliSeq™ Exome RDY S5 Kit allows for rapid, accurate sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.

Designed with leading disease researchers, Ion AmpliSeq™ community panels provide the ability to selectively analyze gene content, as well as the possibility to customize any predesigned community panel to meet your specific research needs.

The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit is ideal for traditional gene expression analysis of known transcripts from the RefSeq database.

Ion Torrent targeted sequencing workflow

Select targets

Construct library

Prepare template

Run sequence

Analyze data

Description: select targets

Description: prepare template

Select from our predesigned gene panels or design your own with the Ion AmpliSeq Designer tool.

Minimize hands-on time with automated Ion AmpliSeq library construction on the Ion Chef™ System.

Hands-on time:
15 minutes

Conveniently go from automated library preparation to automated template preparation and chip loading, all on the versatile Ion Chef System.

Hands-on time:
15 minutes

Get sequence data in as little as 2.5 hours using either the Ion PGM or Ion S5 sequencers.

Hands-on time: As little as 15 minutes

Automate data analysis with Torrent Suite™ Software and find the biological meaning of your data with Ion Reporter™ Software.