Breakout tools for standout work

Get ultimate flexibility and speed for pivotal results with Ion AmpliSeq panels

When navigating through the complexities of genetic diseases, you need a discovery tool that is both flexible and rapid for analyzing variants of interest. Ion AmpliSeq technology can help.

Ion AmpliSeq technology is globally recognized as the gold-standard, amplicon-based enrichment method for targeted next-generation sequencing (NGS). For the fastest, simplest, and most scalable NGS solution, there is no substitute.

Available in Ready-to-Use, Made-to-Order, and On-Demand formats, Ion AmpliSeq panels consist of a pool of oligonucleotide primer pairs, with each pair designed to amplify a specified genomic region. Each panel can be designed to interrogate all bases across a gene, or it can be focused on specific mutation hotspots.

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Gene panels on demand, how and when you want them

Ion AmpliSeq On-Demand panels bring smaller pack sizes and an optimized catalog of pre-tested genes to custom NGS panels, so you can iterate panel design for human disease research with efficiency and convenience.

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Not working with human research samples? No problem.

Design a custom panel from 13 predefined genomes, or from your own reference genome after uploading a reference file.


One tube for CNV, SNP, indel, and fusion detection

Simple, versatile technology based on highly multiplexed PCR helps you target tens to hundreds of genes across mutation types including SNPs, indels, fusions, and copy number variants.


What can you do with NGS using Ion AmpliSeq panels? Get results.

After more than 25,000 custom designs and 1000 publications, custom Ion AmpliSeq panels have a track record of superior coverage uniformity, reproducibility, and specificity. They help you get results.


Need even higher sensitivity? 

To design your own panels and find variants with a very low limit of detection (down to 0.1%), try Ion AmpliSeq HD technology. This novel library amplification technology enables researchers to get answers when ultrahigh sensitivity is required, such as when detecting low-frequency alleles in circulating tumor DNA or trace pathogenic microbial species in blood.

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Ion AmpliSeq panels

On-Demand panels

Ion AmpliSeq On-Demand panels enable custom panel design in smaller pack sizes from pre-tested genes for practical customization that helps reduce upfront cost and risk.

RNA-seq panels

Choose from over 20,000 genes to survey gene expression fold changes starting with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA.

Made-to-order

For maximum flexibility, simply select a subset of targets and enter your specific genomic content online using the Ion AmpliSeq Designer tool, and we will design and create customized Ion AmpliSeq panels just for you. Choose from a number of reference genomes or upload your own reference sequence.

Transcriptome panels

The Ion AmpliSeq Transcriptome Human Gene Expression Kit is ideal for traditional gene expression analysis of known transcripts from the RefSeq database.

Ready-to-use panels

The convenient predesigned Ion AmpliSeq ready-to-use panels allow researchers to focus on data generation and analysis, not on the labor-intensive primer design and target selection steps.

Exome panels

The Ion AmpliSeq Exome RDY S5 Kit allows for rapid, accurate sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.


Ion AmpliSeq panels by application

Deafness, epilepsy, cardiac research, and more

Cancer genomics, cancer RNA expression, and more

Tracing the origin of outbreaks, food safety, and more

DNA phenotyping, ancestry, and more

Power and speed for targeted NGS analysis

Ion AmpliSeq panels help you get NGS results from more of your challenging FFPE samples. To do it faster, use the Ion GeneStudio S5 Series sequencers.


Ion AmpliSeq panel results from Boston Children’s Hospital, USA

Applications of targeted NGS panels for clinical research of primary immunodeficiency diseases 

Dr. Janet Chou and Wayne Bainter discuss the application of the Ion AmpliSeq Primary Immune Deficiency (PID) Research Panel v2 to accelerate and improve the search for causal variants in clinical research of PIDs.

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Videos to help you get started

How does Ion AmpliSeq technology work? 

Transitioning from Sanger sequencing? It’s easy to get started.

Visistat reference component