Whole transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.  With a dynamic range to detect subtle changes in expression level in a hypothesis neutral environment, next generation sequencing enables the understanding of biological response to stimuli or environmental changes.

Step-by-step guide to whole transcriptome analysis sample prep products

PureLink RNA Mini Kit

Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types.

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RiboMinus RNA-Seq kits

Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.

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Ion Total RNA-Seq Kit v2

The Ion Total RNA-Seq Kit v2 provides a fast, simple workflow with as little as 100 ng of total RNA input, while maintaining strand orientation and minimizing bias and error.

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Ion Xpress RNA-Seq Barcode 1-16 Kit

Ion Xpress RNA-Seq Barcode 1-16 Kit provides a set of 16 unique barcode primers for sequencing RNA on the Ion Torrent PGM semiconductor sequencer. When used in combination with the Ion Total RNA-Seq Kit v2, the Ion Xpress RNA-Seq Barcode 1-16 Kit enables users to pool up to 16 fragment libraries prior to template preparation and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for small and whole transcriptome RNA.

PureLink RNA Mini Kit

Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types.

Learn more ›

RiboMinus RNA-Seq kits

Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.

Learn more ›

Ion Total RNA-Seq Kit v2

The Ion Total RNA-Seq Kit v2 provides a fast, simple workflow with as little as 100 ng of total RNA input, while maintaining strand orientation and minimizing bias and error.

Learn more ›

Ion Xpress RNA-Seq Barcode 1-16 Kit

Ion Xpress RNA-Seq Barcode 1-16 Kit provides a set of 16 unique barcode primers for sequencing RNA on the Ion Torrent PGM semiconductor sequencer. When used in combination with the Ion Total RNA-Seq Kit v2, the Ion Xpress RNA-Seq Barcode 1-16 Kit enables users to pool up to 16 fragment libraries prior to template preparation and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for small and whole transcriptome RNA.

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