The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate induced pluripotent stem cells (iPSCs) with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research.

Our stem cell research products have been an integral part of iPSC research from the initial discovery of iPSCs to current breakthroughs. Our broad technology platform provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation.

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Step-by-step guide to iPSC research products

Reprogramming of somatic cells for induced pluripotent stem cell (iPS cell) generation can be accomplished by a number of technologies. We provide traditional and non-integrating tools for iPSC generation from a range of somatic cell types.

Induced pluripotent stem cell (iPSC) research requires careful attention to culture conditions. Gibco media and reagents have been at the forefront of pluripotent stem cell research for years. From KnockOut Serum Replacement and Essential 8 Medium to cell therapy research media, optimized passaging reagents, and substrates, Gibco products are designed to deliver culture with confidence.

Engineer your induced pluripotent stem cells to analyze gene and protein expression and study differentiation and disease pathways. Choose from lipid and electroporation-based transfection products and a range of cloning technologies, including Gateway and TOPO products.

We offer a number of high-quality growth factors and cytokines for targeted differentiation of induced pluripotent stem cells (iPSCS cells). These proteins are extensively tested to help ensure high biological activity, high purity, freeze/thaw stability, and structural homogeneity. In addition, our large portfolio of cell culture media and reagents, along with our custom media services, allows optimal growth, expansion, and storage of differentiated cells.

Validating iPSCs cells to ensure they are pluripotent or have differentiated into the desired cell lineage is a critical step in your research. Invitrogen phosphatase detection kits and cell surface antibodies can assay for pluripotency. Applied Biosystems TaqMan® Array Stem Cell Pluripotency Cards simultaneously analyze expression levels for 96 genes by quantitative PCR, and TaqMan® Protein Assays enable relative quantification of stem cell protein markers from limited quantities of stem cells.