Gibco PSC Media are manufactured according to cGMP for medical devices, 21 CFR Part 820
The original Essential 8 Medium
Many feeder-free stem cell media contain 20 or more components in their formulations. While these media may adequately grow and maintain PSCs, these additional components can introduce unwanted variability, and these formulations commonly exhibit lot-to-lot inconsistencies.
By removing undefined proteins (such as BSA) and other components and including only the ingredients necessary for PSC culture, Essential 8 Medium helps minimize variability in culture. Additionally, Essential 8 Medium is manufactured under cGMP conditions, a further safeguard against lot-to-lot variability that minimizes the risk of variable outcomes and unintended cell differentiation.
Routine PSC expansion and maintenance
Proven to maintain pluripotency in multiple iPSC lines, Essential 8 Medium has been used to scale up production of iPSCs—supporting iPSC growth for >50 passages with no signs of karyotypic abnormalities and maintaining the ability of iPSCs to differentiate into all three germ line lineages.
iPSCs grown on Essential 8 Medium maintain pluripotency and retain normal karyotype. CDI iPSC line shown here at p18 on Essential 8 Medium and Vitronectin.