PSC research requires careful attention to culture conditions. From KnockOut™ media to Essential 8™ media and cell therapy research media, Gibco™ media deliver culture with confidence. Find the right solution for your stem cell culture needs.

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Which pluripotent stem cell culture medium is right for you?

Feeder-based culture of mouse, human, and other mammalian PSCs using a more-defined FBS-free formulation Feeder-free culture with flexibility to optimize bFGF concentration and maintain differentiation capacity of PSCs Optimal feeder-free culture of all human ESC and iPSC lines with minimal lot-to-lot variability All the benefits of Essential 8, but with the added flexiblity of being able to skip weekend media feeds without impacting pluripotency Feeder-based, xeno-free culture of human ESCs and iPSCs offering complete regulatory documentation
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Product name KnockOut™ Serum Replacement - Multi-Species StemPro™ hESC SFM Essential 8™ Medium Essential™ 8 Flex Medium CTS™ KnockOut™ SR XenoFree Medium
Cat. No. A3181502A1000701A1517001A285850112618013
Recommended applications Feeder-based culture; feeder-based reprogramming; PSC derivation, differentiation of PSCs Feeder-free culture Feeder-free, xeno-free culture; feeder-free reprogramming Feeder-free, xeno-free culture, feeder-free reprogrammingFeeder-based, xeno-free culture for cell therapy research
Recommended cell types Mammalian PSCs Human PSCs Human PSCs Human PSCs Human PSCs
Lot-to-lot variability Medium Medium Low Low Medium
Cell morphology Compact colonies with smooth edges; high nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges
Components/unit 1 x 500 mL KnockOut Serum Replacement—Multi-Species 1 x 10 mL StemPro supplement 1 x 500 mL DMEM/F-12 with GlutaMax supplement 1 x 40 mL 25% BSA 1 x 10 mL Essential 8 Supplement
1 x 500 mL DMEM/F-12
1 x 10 mL Essential 8 Flex Supplement 1 x 500 mL DMEM/F12 Essential 8 Flex Basal Medium 1 x 100 mL CTS KnockOut SR XenoFree Medium
Additional components required for complete medium KnockOut DMEM or DMEM/F-12, LIF or bFGF, GlutaMAX-I, NEAA, 2-mercaptoethanol bFGF and 2-mercaptoethanol No additional components required No additional components required bFGF CTS™ and 2-mercaptoethanol
Stability of complete medium Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks
Suitable for cell therapy research
Available resources
Protocols for culturing PSCs, protocols for EB formation

Protocols for culturing PSCs

Protocols for culturing PSCs, FAQs, hands-on training

Protocols for culturing PSCs, FAQs, product documentation

Protocols for culturing PSCs, FAQs, hands-on training