Pluripotent Stem Cell (PSC) Culture Media
4 Media Options, 20+ Protocols, and 50 Years of Innovation
Pluripotent stem cell (PSC) research requires careful attention to culture conditions. To find the right solution for the right environment, tap into peer-reviewed publications, protocols, instructional videos and stem cell experts.
From KnockOut™ Serum Replacement to Essential 8™ Medium and cell therapy research media, Gibco® products deliver culture with confidence.
Which Pluripotent stem cell culture medium is right for you?
|Feeder-based culture of mouse, human, and other mammalian PSCs using a more-defined FBS-free formulation||TFeeder-based culture of PSCs with a widely cited FBS-free formulation offering regulatory documentation||Feeder-free culture with flexibility to optimize bFGF concentration and maintain differentiation capacity of PSCs||Optimal feeder-free culture of all human ESC and iPSC lines with minimal lot-to-lot variability||Feeder-based, xeno-free culture of human ESCs and iPSCs offering complete regulatory documentation|
|Order now||Order now||Order now||Order now||Order now|
|Product name||KnockOut™ Serum Replacement - Multi-Species||KnockOut™ Serum Replacement||StemPro™ hESC SFM||Essential 8™ Medium||CTS™ KnockOut™ XenoFree ESC/iPSC Kit|
|Recommended applications||Feeder-based culture; feeder-based reprogramming; PSC derivation, differentiation of PSCs||Feeder culture; reprogramming on feeders; differentiation of PSCs||Feeder-free culture||Feeder-free, xeno-free culture; feeder-free reprogramming||Feeder-based, xeno-free culture for cell therapy research|
|Recommended cell types||Mammalian PSCs||PSCs||Human PSCs||Human PSCs||Human PSCs|
|Cell morphology||Compact colonies with smooth edges; high nuclear to cytoplasmic ratio||Compact colonies with smooth edges. High nuclear to cytoplasmic ratio||Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges||Compact colonies with smooth edges. High nuclear to cytoplasmic ratio||Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges|
|Components/unit||1 x 500 mL KnockOut Serum Replacement—Multi-Species||1 x 500 mL KnockOut Serum Replacement—Multi-Species||1 x 10 mL StemPro supplement 1 x 500 mL DMEM/F-12 with GlutaMax supplement 1 x 40 mL 25% BSA||1 x 10 mL Essential 8 Supplement
1 x 500 mL DMEM/F-12 with GlutaMax supplement
|1 x 100 mL CTS KnockOut SR XenoFree Medium|
|Additional components required for complete medium||KnockOut DMEM or DMEM/F-12, LIF or bFGF, GlutaMAX-I, NEAA, 2-mercaptoethanol||DMEM/F-12, bFGF, GlutaMAX-I , NEAA, 2-mercaptoethanol||bFGF and 2-mercaptoethanol||No additional components required||bFGF CTS™ and 2-mercaptoethanol|
|Price/500 ml of complete medium||$||$||$$$$||$$$||$$|
|Stability of complete medium||Stable at 4°C for 2 weeks||Stable at 4°C for 2 weeks||Stable at 4°C for 2 weeks||Stable at 4°C for 2 weeks||Stable at 4°C for 2 weeks|
|Suitable for cell therapy research||✓||✓|
Protocols for culturing PSCs, protocols for EB formation
Protocols for culturing PSCs, publications, hands-on training, product finder
Protocols for culturing PSCs
Protocols for culturing PSCs, FAQs, hands-on training
Protocols for culturing PSCs, FAQs, product documentation