4 Media Options, 20+ Protocols, and 50 Years of Innovation

Pluripotent stem cell (PSC) research requires careful attention to culture conditions. To find the right solution for the right environment, tap into peer-reviewed publications, protocols, instructional videos and stem cell experts.

From KnockOut™ Serum Replacement to Essential 8™ Medium and cell therapy research media, Gibco® products deliver culture with confidence.

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Which Pluripotent stem cell culture medium is right for you?

Feeder-based culture of mouse, human, and other mammalian PSCs using a more-defined FBS-free formulation TFeeder-based culture of PSCs with a widely cited FBS-free formulation offering regulatory documentation Feeder-free culture with flexibility to optimize bFGF concentration and maintain differentiation capacity of PSCs Optimal feeder-free culture of all human ESC and iPSC lines with minimal lot-to-lot variability Feeder-based, xeno-free culture of human ESCs and iPSCs offering complete regulatory documentation
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Product name KnockOut™ Serum Replacement - Multi-Species KnockOut™ Serum Replacement StemPro™ hESC SFM Essential 8™ Medium CTS™ KnockOut™ XenoFree ESC/iPSC Kit
Cat. No. A318150210828028A1000701A151700112618013
Recommended applications Feeder-based culture; feeder-based reprogramming; PSC derivation, differentiation of PSCs Feeder culture; reprogramming on feeders; differentiation of PSCs Feeder-free culture Feeder-free, xeno-free culture; feeder-free reprogramming Feeder-based, xeno-free culture for cell therapy research
Recommended cell types Mammalian PSCs PSCs Human PSCs Human PSCs Human PSCs
Lot-to-lot variability Medium Medium Medium Low Medium
Cell morphology Compact colonies with smooth edges; high nuclear to cytoplasmic ratio Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges
Components/unit 1 x 500 mL KnockOut Serum Replacement—Multi-Species 1 x 500 mL KnockOut Serum Replacement—Multi-Species 1 x 10 mL StemPro supplement 1 x 500 mL DMEM/F-12 with GlutaMax supplement 1 x 40 mL 25% BSA 1 x 10 mL Essential 8 Supplement
1 x 500 mL DMEM/F-12 with GlutaMax supplement
1 x 100 mL CTS KnockOut SR XenoFree Medium
Additional components required for complete medium KnockOut DMEM or DMEM/F-12, LIF or bFGF, GlutaMAX-I, NEAA, 2-mercaptoethanol DMEM/F-12, bFGF, GlutaMAX-I , NEAA, 2-mercaptoethanol bFGF and 2-mercaptoethanol No additional components required bFGF CTS™ and 2-mercaptoethanol
Price/500 ml of complete medium $ $ $$$$ $$$ $$
Stability of complete medium Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks
Suitable for cell therapy research
Available Resources
Protocols for culturing PSCs, protocols for EB formation

Protocols for culturing PSCs, publications, hands-on training, product finder

Protocols for culturing PSCs

Protocols for culturing PSCs, FAQs, hands-on training

Protocols for culturing PSCs, FAQs, product documentation
Price/unit $ $ $$$$ $$$ $$