Global gene expression profile assays

PluriTest-compatible PrimeView Global Gene Expression Profile Assays enable quick and cost-effective verification of pluripotency profiling. The technology leverages PrimeView gene expression data in combination with the PluriTest analysis tool, which is a well-established method for verifying pluripotency with over 16,000 samples analyzed. For each sample, more than 36,000 transcripts and variants are compared against an extensive reference set of more than 450 samples, allowing for a direct and detailed comparison of your submitted sample data to hundreds of previously characterized data sets.

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The PrimeView Assays offer:

  • Compatibility with the PluriTest Analysis Tool, a published and established method with over 16,000 samples analyzed
  • Cost-effective confirmation of pluripotency
  • Free and simple-to-use cloud-based analysis tool

PluriTest bioinformatics assay

The PluriTest Assay compares the transcriptional profile of a sample to an extensive reference set of >450 cell and tissue types: including 223 hESC lines, 41 iPSC lines, somatic cells, and tissues. The online analysis tool confirms pluripotency via two separate scores: Pluripotency and Novelty. The Pluripotency Score is an indication of how strongly a model-based pluripotency signature is expressed in the samples analyzed. The Novelty Score indicates the general model fit for a given sample. See Nat Methods 8:315 (2011) for more information.

Instrument requirements

PrimeView Global Gene Expression Profile Assay is compatible with GeneChip Scanner 3000.

PrimeView 16 Global Gene Expression Profile Assay is compatible with the GeneTitan Instrument (110V Model 00-0373 and 220V Model 00-0372). See GeneTitan Instrument site prep guide.

Data analysis

The PrimeView Assays include access to a free and simple-to-use cloud-based analysis tool that confirms pluripotency via two separate scores: Pluripotency and Novelty.

See the PluriTest Analysis Tool.