Feeder-dependent culture proven more reliable than FBS

Gibco™ KnockOut™ reagents represent some of the most cited products for culturing human and mouse pluripotent stem cells (PSCs). With over 4,000 peer-reviewed publications covering applications such as PSC culture, differentiation, iPSC generation, and ESC derivation, KnockOut products are trusted for feeder-dependent PSC culture.

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Choose the Gibco KnockOut supplement that best fits your stem cell culture needs

Application Product Benefits
Feeder-dependent: Clinical applications KnockOut™ Serum Replacement (KSR)
  • A defined substitute for serum, meant for the culture of ESCs and iPSCs
  • Cited in over 2,000 publications for various PSC-related applications
  • Helps to reduce the burden of qualifying reagents when transitioning to clinical applications
Feeder-dependent: Research Use Only KnockOut™ Serum Replacement (SR) – Multi-Species
  • The same KSR formulation, meant for the culture of mouse, human, and other mammalian PSCs
  • Generates more compact colony morphology compared to serum
  • Offers better maintenance of undifferentiated PSCs
Xeno-free and feeder-dependent: Clinical applications CTS™ KnockOut™ SR XenoFree Medium
  • A xeno-free formulation that supports the derivation and maintenance of human ESCs and iPSCs
  • Maintains normal morphology, karyotype, and pluripotency gene expression
  • Helps to reduce the burden of qualifying reagents when transitioning to clinical applications

Choose the Gibco KnockOut basal medium that best fits your stem cell culture needs

Application Product Benefits
Feeder-dependent PSC culture KnockOut™ DMEM
  • A chemically defined basal medium designed for ESCs and iPSCs
  • Mimics the low-osmolality environment of mouse embryonic tissues
  • Reduces PSC differentiation, especially when used with KnockOut SR – Multi-Species
Feeder-dependent PSC culture:
Clinical applications
CTS™ KnockOut™ DMEM
  • A chemically defined, low-osmolality basal medium for ESCs and iPSCs
  • Helps to reduce the burden of qualifying reagents when transitioning to clinical applications
Neural stem cell culture KnockOut™ DMEM/F-12
  • A chemically defined, low-osmolality basal medium without HEPES buffer
  • Supports expansion and differentiation of neural stem cells
  • Maintains human ESCs and iPSCs with normal morphology and karyotype
Neural stem cell culture: Clinical applications CTS™ KnockOut™ DMEM/F-12
  • A chemically defined, low-osmolality basal medium for neural stem cells or ESCs and iPSCs
  • Helps to reduce the burden of qualifying reagents when transitioning to clinical applications