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Pluripotent Stem Cell Handbook

Whether you’re new to the field or expanding into new areas, this educational handbook will help guide your pluripotent stem cell research. It offers the latest tools, resources, applications, and more, to support every step of your PSC workflow.

Gibco PSC Culture virtual training

This virtual lab is an introduction to stem cell culture covering many topics including coating wells to culture PSCs, preparing new cultures of PSCS, and follow-up culture and characterizing pluripotency.


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Type Name
Application note Improving the stem cell characterization workflow: better colony identification using live-cell reagents that enable simultaneous detection of positive and negative pluripotency markers 
Application note Lipofectamine 3000 Reagent: Generation and transfection of induced pluripotent stem cells 
Application note Single cell passaging methods 
FAQ Cell Therapy Systems
FAQ CytoTune-iPS 2.0 Sendai Reprogramming Kit and CytoTune EmGFP Sendai Fluorescence Reporter FAQ 
FAQ Epi5 Episomal iPSC Reprogramming Kit FAQ 
FAQ Episomal iPSC Reprogramming Vectors FAQ 
FAQ Essential 8 Flex Medium 
FAQ Essential 8 Medium
FAQ Essential 8 Medium and Vitronectin 
FAQ Laminin-521/Adaptation Kit
FAQ RevitaCell Supplement
FAQ TaqMan hPSC Scorecard Panel FAQ 
Handbook Antibodies for Stem Cell Research Handbook
Handbook Gibco Neurobiology Protocol Handbook
Handbook Pluripotent Stem Cell Handbook
Literature Cell reprogramming tools 
Literature Cell Therapy Systems brochure 
Literature Essential 8 Media brochure 
Literature Improving the stem cell characterization workflow: Confirming differentiation potential via multi-color detection of germ layer markers 
Literature TaqMan hPSC Scorecard Panel and Software 
Newsletter Stem Cell Intel
Protocol 3-Germ Layer Immunocytochemistry Kit 
Protocol A introduction to stem cell maintenance
Protocol Adaptation of hPSCs from different culture systems to Essential 8 Medium or Essential 8 Flex Medium 
Protocol Creation of embryoid bodies from iPSCs using Complete KnockOut Serum Replacement Feeder-Free Medium
Protocol Culturing human embryonic stem cells on MEF-conditioned medium
Protocol Culturing induced pluripotent stem cells (iPSCs) on MEF-conditioned medium
Protocol Culturing PSCs in Essential 8 Medium 
Protocol CytoTune EmGFP Sendai Fluorescence Reporter 
Protocol CytoTune-iPS 2.0 Sendai Reprogramming Kit 
Protocol Derivation of dopaminergic neurons from human embryonic stem cells
Protocol Detecting pluripotency with Alkaline Phosphatase Live Stain 
Protocol Differentiating glial precursor cells into astrocytes and oligodendrocytes
Protocol Differentiating neural stem cells into neurons and glial cells
Protocol Enrichment and replating of PSC-derived cardiomyocytes 
Protocol Epi5 Episomal iPSC Reprogramming Kit 
Protocol Episomal iPSC Reprogramming Vectors 
Protocol Essential 6 Medium 
Protocol Essential 8 Adaptation Kit 
Protocol Essential 8 Flex Medium 
Protocol Essential 8 Medium 
Protocol Feeder-dependent culture and passaging mouse embryonic stem cells (mESCs) in KnockOut Serum Replacement with leukemia inhibitory factor (LIF)
Protocol Feeder-dependent culture of human embryonic stem cells (hESCs)
Protocol Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs)
Protocol Formation of embryoid bodies (EBs) from mouse embryonic stem cells (mESCs)
Protocol Geltrex LDEV-Free, hESC-qualified, Reduced Growth Factor Basement Membrane Matrix 
Protocol General Stem Cell Protocols
Protocol Generation of Human Induced Pluripotent Stem Cells (hiPSC) from Fibroblasts using Episomal Vectors 
Protocol Gibco Episomal hiPSC Line 
Protocol hPSC Scorecard Analysis Software
Protocol Human Cardiomyocyte Immunocytochemistry Kit 
Protocol Human Dopaminergic Neuron Immunocytochemistry Kit 
Protocol Human Neural Stem Cell Immunocytochemistry Kit 
Protocol Induction of neural stem cells from human pluripotent stem cells using Gibco PSC Neural Induction Medium 
Protocol Mouse (ICR) Inactivated Embryonic Fibroblasts 
Protocol Neural Stem Cell Protocols
Protocol Pluripotent Stem Cell Protocols
Protocol PSC 4-Marker Immunocytochemistry Kit (OCT4, SSEA4, SOX2, TRA-1-60) 
Protocol PSC Cardiomyocyte Differentation Kit 
Protocol PSC Cardiomyocyte Differentation Kit quick reference guide 
Protocol PSC Definitive Endoderm Induction Kit 
Protocol PSC Definitive Endoderm Induction Kit quick reference guide 
Protocol PSC Dopaminergic Neuron Differentiation Kit (prototype) 
Protocol PSC Neural Induction Medium 
Protocol PSC Neural Induction Medium quick reference guide 
Protocol Reprogramming peripheral blood mononuclear cells (PBMCs) with the CytoTune-iPS 2.0 Reprogramming Kit under feeder-free conditions 
Protocol RevitaCell Supplement 
Protocol Single-cell dissociation of PSCs for cardiomyocyte differentiation 
Protocol StemPro hESC SFM 
Protocol TaqMan hPSC Scorecard Panel Quick Reference Card 
Protocol Using the TaqMan hPSC Scorecard Panel 
Protocol Vitronectin, truncated recombinant human (VTN-N) 
Publication A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells 
Publication A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific iPSCs 
Publication An efficient method for the derivation of mouse embryonic stem cells 
Publication Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai virus vectors 
Publication Cellular reprogramming of human peripheral blood cells 
Publication Characterization of human Huntington's Disease cell model from induced pluripotent stem cells 
Publication Chemically defined conditions for human iPSC derivation and culture 
Publication Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture  
Publication Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7 
Publication Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor 
Publication Defined hPSC culture enables highly efficient neuroepithelium derivation without small molecule inhibitors 
Publication Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells 
Publication Derivation of induced pluripotent stem cells from pig somatic cells 
Publication Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation 
Publication Disease-specific induced pluripotent stem cells 
Publication Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium 
Publication Efficient feeder-free episomal reprograming with small molecules 
Publication Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord bloodmononuclear cells 
Publication Efficient iPS cell production with the MyoD transactivation domain in serum-free culture 
Publication Electromechanical integration of cardiomyocytes derived from human embryonic stem cells 
Publication Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing 
Publication Feeder layer- and serum-free culture of human embryonic stem cells 
Publication Feeder-free growth of undifferentiated human embryonic stem cells 
Publication Generation of germline-competent rat induced pluripotent stem cells 
Publication Generation of GFP reporter human induced pluripotent stem cells using AAVS1 safe harbor Transicription Activator Like Effector Nuclease 
Publication Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors 
Publication Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts 
Publication Generation of induced pluripotent stem cells in the absence of drug selection 
Publication Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches 
Publication Human dermal stem cells differentiate into functional epidermal melanocytes 
Publication Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells 
Publication Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons 
Publication Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media 
Publication Induced pluripotent stem cell reprogramming by integration-free sendai virus vectors from peripheral blood of patients with craniometaphyseal dysplasia 
Publication Induced pluripotent stem cells for neural tissue engineering 
Publication Induction of pluripotent stem cells from adult human fibroblasts by defined factors 
Publication Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds 
Publication Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 
Publication Isolation of single-base genome-edited human iPS cells with antiobiotic selection 
Publication Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor 
Publication Motor Neuron Differentiation from Pluripotent Stem Cells and Other Intermediate Proliferative Precursors that can be Discriminated by Lineage Specific Reporters 
Publication New cell lines from mouse epiblast share defining features with human embryonic stem cells 
Publication Neural Cell Culture Publications Finder
Publication Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency 
Publication Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse 
Publication Passaging and colony expansion of hPSCs by enzyme-free dissocation in chemically define culture conditions 
Publication Publications citing the Sendai virus for iPSC generation
Publication Reprogramming of human somatic cells to pluripotency with defined factors 
Publication Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors 
Publication RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications 
Publication Scalable expansion of human iPSCs in the defined xeno-free E8 medium under adherent and suspension culture conditions 
Publication Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single-cell analysis 
Publication Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines 
Publication Signaling defects in iPSC-derived fragile X premutation neurons 
Publication Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells 
Publication Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage 
Publication Whole-Genome Sequencing Analysis Reveals High Specificity of CRISPR/Cas9 and TALEN-Based Genome Editing in Human iPSCs 
Scientific poster A Novel Essential 8 Media System for Robust Weekend-Free Culture of Pluripotent Stem Cells 
Scientific poster Antibody-based tools and protocols for improving stem cell characterization workflow 
Scientific poster Cell surface marker expression analysis to track progression of fibroblast reprogramming 
Scientific poster Characterization of human limbal stem cells using acoustic focusing cytometry 
Scientific poster Clinical-grade expansion of human mesenchymal stem cells using a microcarrier-based culture system under serum-free and xeno-free conditions 
Scientific poster CytoTune-iPS 2.0 Sendai Reprogramming Ki for high efficiency reprogramming of fibroblasts and blood-derived cells 
Scientific poster Development of high-quality MSC and NSC for human disease research 
  Direct derivation and prospective isolation of Schwann cell precursors from human pluripotent stem cells 
Scientific poster Differentiating human pluripotent stem cells to neurons - Approaches in media development 
Scientific poster Effect of DGCR8 knockdown on human embryonic and adult stem cells 
Scientific poster Efficient and scalable GMP-grade culture media system for rapid cardiomyocyte differentiation of pluripotent stem cells in human disease research 
Scientific poster Efficient generation and characterization of neural stem cells derived from Parkinson’s disease patient and age-matched control induced pluripotent stem cell lines using a novel neural induction method 
Scientific poster Efficient generation of induced pluripotent stem cells derived from Parkinson's disease study patient fibroblast lines using CytoTune-iPS 2.0 Reprogramming Kit in the Essential feeder-free media system 
Scientific poster Efficient induction and scale up generation of neural stem cells from human pluripotent stem cells 
Scientific poster Efficient method to generate vector-free induced pluripotent stem cells from CD34+ and PBMCs using the CytoTune iPS Sendai Reprogramming Kit 
Scientific poster Enabling successful reprogramming of peripheral blood mononuclear cells to induced pluripotent stem cells with CytoTune-iPS 2.0 Sendai Reprogramming Kit 
Scientific poster Essential 8 Medium: A xeno-free, feeder-free medium for the culture and generation of human iPSCs 
Scientific poster Essential media system for reprogramming 
Scientific poster Generation of transgene-free iPSCs From Parkinson’s disease patients 
Scientific poster Generation of zero-footprint iPSCs in xeno-free or feeder-free with CytoTune™-iPS Sendai Reprogramming Kit 
Scientific poster Genome Editing of α-Synuclein in iPSCs from a Donor with Multiple System Atrophy 
Scientific poster Genome editing of induced pluripotent stem cells from Parkinson’s disease and multiple system atrophy patients 
Scientific poster High-quality cGMP-manufactured human mesenchymal stem cell and neural stem cell for human disease research 
Scientific poster High-quality episomal human iPSC derived from cord blood with a zero footprint: Applications in serum-free and xeno-free culture 
Scientific poster Highly efficient and robust generation of neural stem cells from human pluripotent stem cells 
Scientific poster Human adipose-derived stem cell characterization and comparative gene expression profiling 
Scientific poster Improved cryopreservation and recovery solutions for pluripotent stem cells and difficult-to-preserve primary cells 
Scientific poster Integrated platform for creation of assay-ready neural cells 
Scientific poster Large-scale and focused transcriptome analysis of integration-free induced pluripotent stem cells 
Scientific poster Lipofectamine 3000: A new transfection reagent for iPSC generation and stem cell genomic engineering 
Scientific poster Modulating growth factor addition to Essential 6 media for a complete xeno-free media from fibroblast culture to iPSC generation and expansion 
Scientific poster Molecular analysis tools for epigenomic characterization of pluripotent stem cells 
Scientific poster Novel Alkaline Phosphatase Live Stain to Identify and Isolate Emerging iPSCs 
Scientific poster Novel markers to distinguish pluripotent and non-pluripotent cells 
Scientific poster Novel neural induction method for efficient generation of neural stem cells dervied from Parkinson's 
Scientific poster Optimized neuronal culture media for electrophysiology 
Scientific poster Process development strategies to enable large-scale expansion of mesenchymal stem cells for cellular therapy 
Scientific poster Quantitation of rare undifferentiated hESCs by methylation-specific CastPCR (MeS castPCR) 
Scientific poster Rapid manufacture of custom TAL effector nucleases for genomic editing 
Scientific poster Synthetic surfaces for the culture of human embryonic and adult stem cells 
Scientific poster Synthetic surfaces for the culture of human embryonic and adult stem cells 
Scientific poster TaqMan Assays for rapid assessment of genome, epigenome, and quality of intergration free induced pluripotent stem cells 
Scientific poster Use of surrogate reporter vectors to enrich for CRISPR- & TALEN-modified cells 
Scientific poster Using cells from patients with multiple systems atrophy or Parkinson’s disease (PARK2, LRRK2, and/or GBA mutations) to build stem cell-derived disease model systems 
Scientific poster Utility of hPSC Scorecard Assay in assessment of functional pluripotency of cells across the iPSC workflow 
Scientific poster Utilizing advanced cryopreservation techniques to produce assay ready primary mouse neurons 
Scientific poster Xeno-free culture systems for pluripotent stem cells 
Scientific poster Xeno-free culture systems for stem cells 
Selection guide Mesenchymal Stem Cell Resource Guide
Selection guide Tools for neural cell culture of primary cells
Selection guide Neural cell culture systems
Selection guide Pluripotent Stem Cell Guidebook 
Training Gibco PSC Culture Virtual Training Lab
Training Gibco PSC Workshop
Video A Thought Leader's Perspective: Unlocking cardiovascular disease with iPS cells - Dr. Chad Cowan 
Video Advances in Stem Cell Research - Dr. Alexander Meissner 
Video Advances in Stem Cell Research: Essential 8 Medium, A new standard for iPSC cell culture - Dr. Emile Nuwaysir 
Video Alkaline Phosphatase Live Stain 
Video CTS Mini-Documentary Series
Video How to adapt pluripotent stem cells from other PSC media into Essential 8 Medium 
Video How to differentiate pluripotent stem cells into cardiomyocytes 
Video How to induce pluripotent stem cells in to definitive endoderm 
Video How to passage PSCs in Essential 8 Medium 
Video How to prepare Essential 8 Medium for PSC Culture 
Video How to prepare Vitronectin substrate plates for use with Essential 8 Medium 
Video How to use Gibco PSC Neural Induction Medium 
Video How to Use the TaqMan hPSC Scorecard Panel 
Video Why we chose the TaqMan hPSC Scorecard Assay - Jennifer Moore, RUCDR/Infinite Biologics 
Web PSC Dopaminergic Neuron Differentiation Kit (prototype) landing page
Webinar 24 Hours of Stem Cells - On-demand presentations 2014/2015
Webinar Antibody-based tools and protocols for characterization of pluripontent stem cells 
Webinar Antibodies for stem cell research
Webinar Barriers in stem cell therapy - Dr Jeanne Loring 
Webinar Building a stem cell matrix - Dr Jeanne Loring 
Webinar Characterization of human pluripotent stem cells - Dr. Alexander Meissner 
Webinar Considerations for the culture of pluripotent stem cells 
Webinar Creating integration-free iPSCs and NSCs to Model Parkinson's disease - Dr. Birgitt Schuele 
Webinar Culture adaptation of pluripotent stem cells: an in vitro model for oncogensis
Webinar Culture & characterization systems for hESCs and iPSCs
Webinar Culture & characterization systems for mesenchymal stem cells (hMSC)
Webinar Development of chemically defined cell culture for human pluripotent stem cells
Webinar Differentiation of Midbrain Floor Plate Progenitors and Dopaminergic Neurons from Human Pluripotent Stem Cells 
Webinar Eliminate serum to gain control of T cell research - New T cell-defined serum replacement 
Webinar Epigenetic regulation in stem cells and reprogramming
Webinar Essential 8 & Essential 6 - A feeder-free, xeno-free system for reprogramming and culture of PSCs 
Webinar Essential 8 Flex Medium: Eliminating daily feeding in a feeder-free, xeno-free PSC culture system 
Webinar Eyeing the Single Stem Cell - Dr Jeanne Loring 
Webinar Generation of transgene-free induced pluripotent stem cells - Dr Uma Lakshmipathy 
Webinar Genome editing and stem cell engineering for disease modeling
Webinar Highly efficient genome editing and cell engineering in stem cells using CRISPR/Cas9 
Webinar Human neural stem cell (hNSC) platforms
Webinar Improved T cell function and in vivo engraftment of CAR-T cells expanded ex vivo with CTS Immune Cell SR 
Webinar Inducing excitement with pluripotency - Dr Jeanne Loring 
Webinar Identifying iPSCs with a novel Live Alkaline Phosphatase Stain 
Webinar Leaving nothing to chance - Dr Mark Tomishima 
Webinar mircoRNA and epigenetic regulation of neural stem cell differentiations - Dr Ron Hart 
Webinar MSC Culture & Beyond
Webinar MSC Culture Systems & Applications
Webinar Molecular engineering of biomaterial platforms for scalable stem cell manufacturing
Webinar Next-generation genome analysis tools for stem cells - Dr Michael Rhodes 
Webinar Pluripotent stem cell characterization methods 
Webinar Pluripotent Stem Cell Culture Systems: Identification of Appropriate Medium, Matrix, and Passaging Reagent for Your Stem Cell Workflow Needs 
Webinar PSCs to cardiomyocytes in three steps 
Webinar Rapid induction of neural stem cells without the need for EB formation 
Webinar Reprogramming cells to study mental disorders
Webinar Stem cells as a model for morphogenesis of tissues - Dr Elaine Fuchs 
Webinar Stemming the loss of species - Dr Jeanne Loring 
Webinar The evolution of PSC culture media - Dr Rupa Shevde 
Webinar The future of pluripotent stem cell banking
Webinar Thermo Fisher Scientific's cell therapy capabilities 
Webinar Utility of gene expression signatures for the characterization of cellular identity
Webinar Xeno-free derivation and maintenance of pluripotent cell lines - Dr Jordan Pomeroy 
White paper Disease modeling of hypertrophic cardiomyopathy from patients carrying sarcomeric mutations