Quantitative analysis of trilineage differentiation potential

The Applied Biosystems™ TaqMan® hPSC Scorecard™ Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software.

Request more information ›

Sign in to software ›

Quantify trilineage differentiation potential of your PSCs

Why We Chose the TaqMan hPSC Scorecard Assay Jennifer Moore, RUCDR/Infinite Biologics

The hPSC Scorecard assay was developed in collaboration with Alex Meissner, and follows from his landmark publication (Cell 2011 144:439–452). The accompanying analysis software gives researchers anywhere in the world the ability to compare the gene expression profiles of their cell lines to a common reference set.

Instrument requirements


See which instruments are compatible with the TaqMan hPSC Scorecard Panel and how to set them up.

Data analysis


The TaqMan hPSC Scorecard Panel includes access to free, intuitive web-based analysis software that allows researchers to quantitatively compare the gene expression profile of their sample to that of a reference set.

CellModel Services


Lacking the resources or instruments to use the Scorecard Panel? Let us do it for you!

Need help? Have a question?Find resources ›

What is it?

The TaqMan hPSC Scorecard Panels quantitatively confirms pluripotency (trilineage differentiation potential)
for both embryonic and induced pluripotent stem cell lines. The hPSC Scorecard™ Panel (available in 96 and 384-well format) contains 94 predefined TaqMan Gene Expression assays (including endogenous controls) dried-down in assay plate wells. The panel of genes was developed in collaboration with Alex Meissner, and based on his landmark publication (Cell 144:439-452 (2011)).

The TaqMan hPSC Scorecard Panel provides:

  • A quantitative assay for confirming pluripotency and trilineage differentiation potential
  • Comparison of expression profiles to a reference standard
  • An easy to use platform with pre-plated assays and dedicated, intuitive analysis software

Overview of protocol:

  1. Test undifferentiated, spontantious differentiation (EB) or directed (monolayer) differentiation samples
  2. Extract and purify RNA
  3. Generate cDNA
  4. Run gene expression panel on a compatible qRT-PCR instrument 
  5. Upload .eds files to the hPSC Scorecard Analysis Software
  6. Analyze data and export results from the convenient cloud-based analysis application

How to run the hPSC Scorecard Assay

Undifferentiated ESC/iPSC

Differentiated ESC/iPSC: Embryoid bodies (week 1) - or - Directly differentiated cells.

Sample requirements: >1 x 106 cells/sample

RNA Isolation



TRIzol® organic phase extraction

Sample requirements: 1 mL TRIzol® reagent per 6 cm culture dish

[RNA] (mg/mL) = A260 * 40

Recommended A260/A280 ratio = 1.6 – 1.8

Analyze data from the TaqMan® hPSC Scorecard™ Panel using the intuitive analysis software

Sign in to the hPSC Scorecard™ Analysis Software