Let Cells Glow

We want to help you let your cells glow

As leading brands in cell culture, transfection and fluorescence imaging solutions, Invitrogen, Gibco, and Thermo Scientific products help our customers achieve the best fluorescence cell imaging quality. Together, we #LetCellsGlow!
 

On this page:

Adil Mohameds image

Researcher: Adil Mohamed
Institute: University of Alberta

Products used: APEX Alexa Fluor 568 Antibody Labeling KitAlexa Fluor 647 Antibody Labeling Kit

Erik Rodriguezs image

Researcher: Erik Rodriguez
Institute:  The George Washington University

Products used: Lipofectamine 3000 Transfection ReagentB-27 Plus SupplementNeurobasal MediumOpti-MEM I Reduced Serum Medium
Joanna Palades image

Researcher: Joanna Palade
Institute: Arizona State University

Products used: EVOS FL Auto 2 Imaging System

Laura Gorrells image

Researcher: Laura Gorrell
Institute: National Institutes of Health (NIH)

Products used: EVOS FL Auto 2 Imaging System

Sarah Zimmermanns image

Researcher: Sarah Zimmermann
Institute: University of Colorado, Boulder

Products used: ProLong Diamond Antifade Mountant; Alexa Fluor 546 phalloidin conjugate; Goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor Plus 488 conjugate
Henry Lujans image

Researcher: Henry Lujan
Institute: Baylor University

Products used: ProLong Diamond Antifade MountantImage-iT Fixation/Permeabilization Kit
Catherine Millar Haskells image

Researcher: Catherine Millar-Haskell
Institute: University of Delaware

Products used: Alexa Fluor 647 phalloidin conjugate
Bin Nis image

Researcher: Bin Ni
Institute: Virginia Commonwealth University

Products used: MitoTracker Deep Red FM; NucBlue Live ReadyProbes Reagent; BODIPY 493/503
Prathyusha Kondas image

Researcher: Prathyusha Konda
Institute: Dalhousie University

Products used: ProLong Diamond Antifade Mountant; APEX Alexa Fluor 568 Antibody Labeling KitAPEX Alexa Fluor 488 Antibody Labeling Kit
Tierah Hinchliffes image

Researcher: Tierah Hinchliffe
Institute: University of Alberta

Products used: EVOS FL Auto 2 Imaging System

Catherine Millar-Haskells image

Researcher: Catherine Millar-Haskell
Institute: University of Delaware

Products used: Alexa Fluor 647 phalloidin conjugate

Kenneth Darigos image

Researcher: Kenneth D'Arigo
Institute: Iovance Biotherapeutics

Products used: Image-iT Fixation/Permeabilization Kit
Tess Nguyens image

Researcher: Tess Nguyen
Institute: Stanford University

Products used: Donkey anti-goat IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594 conjugate
Beth Worleys image

Researcher: Beth Worley
Institute: Penn State College of Medicine

Products used: Invitrogen MitoTracker Green FM 

Jacqualyn Schulmans image

Researcher: Jacqualyn Schulman
Institute: SUNY Upstate Medical University

Products used: Invitrogen Neon Transfection System

Thermo Scientific fluorescence cell imaging reagents and instruments

B-27 Plus Neuronal Culture System

B-27 Plus Neuronal Culture System is a next-generation media system designed to give highest rate of in vitro survival of primary rodent and human stem cell–derived neurons.

  • At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
  • Improved electrophysiological activity and maturation
  • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Learn more


FluoroBrite DMEM

Gibco FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health.

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Learn more


Fluorescent cell imaging reagents

Fluorescent cell imaging reagents
A 60 sec time course shows the resistance to photobleaching achieved with ProLong Glass antifade mountant. Fixed HeLa cells were labeled with Invitrogen Fluorescein Phalloidin and mounted in ProLong Glass antifade mountant or 50% phosphate-buffered saline (PBS)/glycerol solution. Images were acquired at 12 sec intervals using a 20x objective with continuous illumination from a standard 100 W Hg-arc lamp.

We offer innovative Invitrogen fluorescent reagents for cell and protein labeling that can help you get publication-quality images easily, the first time.

Reagents for 5-Steps to Publication-Quality Images of Fixed Cells
Reagents for Live-Cell Imaging


Dishes and plates for high-quality imaging

Thermo Scientific Nunc glass-bottom dishes—a polystyrene fixture combined with a glass-coverslip bottom offers the optimum optical characteristics required for high-magnification microscopy and confocal image analysis.

Thermo Scientific Nunc 96- and 384-well optical-bottom plates—cell culture–treated coverglass-based plates available with several surface-treatment options and are designed to deliver optimum clarity with minimum light scatter and autofluorescence background.

Thermo Scientific Nunc Lab-Tek Chamber Slide and Chambered Coverglass—seed, incubate, fix, and stain on a single microscope slide using the chamber slide system with removable chamber; ideal for high-magnification microscopy and confocal image analysis.


Lipofectamine reagent family

Lipofectamine reagent family
Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours posttransfection. Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines.

Invitrogen Lipofectamine transfection reagents are designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures.


Neon Transfection System

Invitrogen Neon Transfection System is a simple, customizable, and gentle electroporation instrument designed for high transfection efficiency

  • Superior results—effective in difficult cells, including T and B cells
  • Customizable—preprogrammed 24-well optimization protocols and open platform for additional protocols
  • Simple—the pipette based system is easy to use allows for increased cell viability compared to traditional cuvette based systems

Learn more

Invitrogen antibodies

Count on Invitrogen antibodies, verified with our 2-part testing approach to help ensure consistent results and reproducibility. We offer more than 83,000 primary and secondary antibodies covering over 91% of the humane proteome. 

Search all antibodies

SuperBright primary antibodies

Invitrogen eBioscience Super Bright antibodies are a superior fluorophore choice for your flow cytometer’s violet laser. They have exceptional brightness and photostability, low background, and allow for greater utilization of the violet laser by enabling detection channels from 436 nm to 702 nm. For cells with low expressed target proteins, these bright reagents can provide the difference to detect and resolve dim populations.

Learn more


Alexa Fluor Plus secondary antibodies

Alexa Fluor Plus secondary antibodies were designed to help you detect and visualize low-abundance targets in rare or precious samples. The unique proprietary “plus” dye chemistry coupled with high pre-adsorption offers up to 4x higher signal-to-noise, minimizes cross-reactivity and yields increased sensitivity to provide more detail.

Learn more

Countess II FL Automated Cell Counter

A benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability quickly and easy.

Learn more


EVOS FL Auto 2 Imaging System

Supports high-resolution mosaic tiling, Z-stacking, multi-position well scanning, cell counting with thresholding, live cell imaging, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage, and high quality optics enable you to produce publication-quality images in seconds.

Learn more


CellInsight CX7 and CX7 LZR High-Content Analysis (HCA) Platforms

Thermo Scientific CellInsight CX7 and CX7 LZR HCA Platforms offer an integrated way to develop and automate high-content assays using a combination of bright-field and 7-color fluorescent imaging with LEDs or lasers in confocal or wide-field modes. Achieve quantitative, multiparameter cell imaging and analysis simultaneously with HCA.

Learn more

Varioskan LUX Multimode Microplate Reader

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including fluorescence, absorbance, luminescence, AlphaScreen tools, and time-resolved fluorescence.

Learn more


Attune NxT Flow Cytometer

The Attune NxT Flow Cytometer system enables multiparametric flow cytometry to both new and experienced researchers. Using acoustic assisted hydrodynamic focusing technology to accelerate experimental run times, this compact flow cytometer offers rapid and accurate analysis for a broad range of sample types including fluorescent-labeled cells.

  • Efficient—up to 10X faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors; up to 6 channels off the violet laser
  • Transformative—run difficult samples with a system that is less sensitive to clogging

Learn more

Haven’t tried our most popular imaging reagents and instruments yet?  We provide sampling and DEMO programs so you can see how amazing they are before you buy it!

Sampling program

Demo program

B-27 Plus Neuronal Culture System

B-27 Plus Neuronal Culture System is a next-generation media system designed to give highest rate of in vitro survival of primary rodent and human stem cell–derived neurons.

  • At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
  • Improved electrophysiological activity and maturation
  • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Learn more


FluoroBrite DMEM

Gibco FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health.

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Learn more


Fluorescent cell imaging reagents

Fluorescent cell imaging reagents
A 60 sec time course shows the resistance to photobleaching achieved with ProLong Glass antifade mountant. Fixed HeLa cells were labeled with Invitrogen Fluorescein Phalloidin and mounted in ProLong Glass antifade mountant or 50% phosphate-buffered saline (PBS)/glycerol solution. Images were acquired at 12 sec intervals using a 20x objective with continuous illumination from a standard 100 W Hg-arc lamp.

We offer innovative Invitrogen fluorescent reagents for cell and protein labeling that can help you get publication-quality images easily, the first time.

Reagents for 5-Steps to Publication-Quality Images of Fixed Cells
Reagents for Live-Cell Imaging


Dishes and plates for high-quality imaging

Thermo Scientific Nunc glass-bottom dishes—a polystyrene fixture combined with a glass-coverslip bottom offers the optimum optical characteristics required for high-magnification microscopy and confocal image analysis.

Thermo Scientific Nunc 96- and 384-well optical-bottom plates—cell culture–treated coverglass-based plates available with several surface-treatment options and are designed to deliver optimum clarity with minimum light scatter and autofluorescence background.

Thermo Scientific Nunc Lab-Tek Chamber Slide and Chambered Coverglass—seed, incubate, fix, and stain on a single microscope slide using the chamber slide system with removable chamber; ideal for high-magnification microscopy and confocal image analysis.


Lipofectamine reagent family

Lipofectamine reagent family
Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours posttransfection. Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines.

Invitrogen Lipofectamine transfection reagents are designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures.


Neon Transfection System

Invitrogen Neon Transfection System is a simple, customizable, and gentle electroporation instrument designed for high transfection efficiency

  • Superior results—effective in difficult cells, including T and B cells
  • Customizable—preprogrammed 24-well optimization protocols and open platform for additional protocols
  • Simple—the pipette based system is easy to use allows for increased cell viability compared to traditional cuvette based systems

Learn more

Invitrogen antibodies

Count on Invitrogen antibodies, verified with our 2-part testing approach to help ensure consistent results and reproducibility. We offer more than 83,000 primary and secondary antibodies covering over 91% of the humane proteome. 

Search all antibodies

SuperBright primary antibodies

Invitrogen eBioscience Super Bright antibodies are a superior fluorophore choice for your flow cytometer’s violet laser. They have exceptional brightness and photostability, low background, and allow for greater utilization of the violet laser by enabling detection channels from 436 nm to 702 nm. For cells with low expressed target proteins, these bright reagents can provide the difference to detect and resolve dim populations.

Learn more


Alexa Fluor Plus secondary antibodies

Alexa Fluor Plus secondary antibodies were designed to help you detect and visualize low-abundance targets in rare or precious samples. The unique proprietary “plus” dye chemistry coupled with high pre-adsorption offers up to 4x higher signal-to-noise, minimizes cross-reactivity and yields increased sensitivity to provide more detail.

Learn more

Countess II FL Automated Cell Counter

A benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability quickly and easy.

Learn more


EVOS FL Auto 2 Imaging System

Supports high-resolution mosaic tiling, Z-stacking, multi-position well scanning, cell counting with thresholding, live cell imaging, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage, and high quality optics enable you to produce publication-quality images in seconds.

Learn more


CellInsight CX7 and CX7 LZR High-Content Analysis (HCA) Platforms

Thermo Scientific CellInsight CX7 and CX7 LZR HCA Platforms offer an integrated way to develop and automate high-content assays using a combination of bright-field and 7-color fluorescent imaging with LEDs or lasers in confocal or wide-field modes. Achieve quantitative, multiparameter cell imaging and analysis simultaneously with HCA.

Learn more

Varioskan LUX Multimode Microplate Reader

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including fluorescence, absorbance, luminescence, AlphaScreen tools, and time-resolved fluorescence.

Learn more


Attune NxT Flow Cytometer

The Attune NxT Flow Cytometer system enables multiparametric flow cytometry to both new and experienced researchers. Using acoustic assisted hydrodynamic focusing technology to accelerate experimental run times, this compact flow cytometer offers rapid and accurate analysis for a broad range of sample types including fluorescent-labeled cells.

  • Efficient—up to 10X faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors; up to 6 channels off the violet laser
  • Transformative—run difficult samples with a system that is less sensitive to clogging

Learn more

Haven’t tried our most popular imaging reagents and instruments yet?  We provide sampling and DEMO programs so you can see how amazing they are before you buy it!

Sampling program

Demo program

Want amazing images like this?

Get tips & tricks in the Molecular Probes School of Fluorescence
Free guide – Five steps for publication-quality cell imaging
Contact our technical support specialists

These images were captured using the EVOS FL Auto 2 Imaging System.

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