Let Cells Glow
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As leading brands in cell culture, transfection and fluorescence imaging solutions, Invitrogen, Gibco, and Thermo Scientific products help our customers achieve the best fluorescence cell imaging quality.  Browse the sections below to see the reagents and instruments we’ve developed to help you achieve superior imaging results.
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These images were captured using the EVOS FL Auto 2 Imaging System.

Thermo Scientific fluorescence cell imaging reagents and instruments

As leading brands in cell culture, transfection and fluorescence imaging solutions, Invitrogen, Gibco, and Thermo Scientific products help our customers achieve the best fluorescence cell imaging quality. Browse the sections below to see the reagents and instruments we’ve developed to help you achieve superior imaging results.

B-27 Plus Neuronal Culture System

B-27 Plus Neuronal Culture System is a next-generation media system designed to give highest rate of in vitro survival of primary rodent and human stem cell–derived neurons.

  • At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
  • Improved electrophysiological activity and maturation
  • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Learn more


FluoroBrite DMEM

Gibco FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health.

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Learn more


Fluorescent cell imaging reagents

Fluorescent cell imaging reagents
A 60 sec time course shows the resistance to photobleaching achieved with ProLong Glass antifade mountant. Fixed HeLa cells were labeled with Invitrogen Fluorescein Phalloidin and mounted in ProLong Glass antifade mountant or 50% phosphate-buffered saline (PBS)/glycerol solution. Images were acquired at 12 sec intervals using a 20x objective with continuous illumination from a standard 100 W Hg-arc lamp.

We offer innovative Invitrogen fluorescent reagents for cell and protein labeling that can help you get publication-quality images easily, the first time.

Reagents for 5-Steps to Publication-Quality Images of Fixed Cells
Reagents for Live-Cell Imaging


Dishes and plates for high-quality imaging

Thermo Scientific Nunc glass-bottom dishes—a polystyrene fixture combined with a glass-coverslip bottom offers the optimum optical characteristics required for high-magnification microscopy and confocal image analysis.

Thermo Scientific Nunc 96- and 384-well optical-bottom plates—cell culture–treated coverglass-based plates available with several surface-treatment options and are designed to deliver optimum clarity with minimum light scatter and autofluorescence background.

Thermo Scientific Nunc Lab-Tek Chamber Slide and Chambered Coverglass—seed, incubate, fix, and stain on a single microscope slide using the chamber slide system with removable chamber; ideal for high-magnification microscopy and confocal image analysis.


Opti-MEM Reduced Serum Medium

Gibco Opti-MEM I Reduced-Serum Medium (MEM) is an improved MEM that allows for a reduction of fetal bovine serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine reagents.

Learn more

Lipofectamine reagent family

Lipofectamine reagent family
Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours posttransfection. Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines.

Invitrogen Lipofectamine transfection reagents are designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures.


Neon Transfection System

Invitrogen Neon Transfection System is a simple, customizable, and gentle electroporation instrument designed for high transfection efficiency

  • Superior results—effective in difficult cells, including T and B cells
  • Customizable—preprogrammed 24-well optimization protocols and open platform for additional protocols
  • Simple—the pipette based system is easy to use allows for increased cell viability compared to traditional cuvette based systems

Learn more

Invitrogen antibodies

Count on Invitrogen antibodies, verified with our 2-part testing approach to help ensure consistent results and reproducibility. We offer more than 83,000 primary and secondary antibodies covering over 91% of the humane proteome. 

Search all antibodies

SuperBright primary antibodies

Invitrogen eBioscience Super Bright antibodies are a superior fluorophore choice for your flow cytometer’s violet laser. They have exceptional brightness and photostability, low background, and allow for greater utilization of the violet laser by enabling detection channels from 436 nm to 702 nm. For cells with low expressed target proteins, these bright reagents can provide the difference to detect and resolve dim populations.

Learn more


Alexa Fluor Plus secondary antibodies

Alexa Fluor Plus secondary antibodies were designed to help you detect and visualize low-abundance targets in rare or precious samples. The unique proprietary “plus” dye chemistry coupled with high pre-adsorption offers up to 4x higher signal-to-noise, minimizes cross-reactivity and yields increased sensitivity to provide more detail.

Learn more

Countess II FL Automated Cell Counter

A benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability quickly and easy.

Learn more


EVOS FL Auto 2 Imaging System

Supports high-resolution mosaic tiling, Z-stacking, multi-position well scanning, cell counting with thresholding, live cell imaging, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage, and high quality optics enable you to produce publication-quality images in seconds.

Learn more


CellInsight CX7 and CX7 LZR High-Content Analysis (HCA) Platforms

Thermo Scientific CellInsight CX7 and CX7 LZR HCA Platforms offer an integrated way to develop and automate high-content assays using a combination of bright-field and 7-color fluorescent imaging with LEDs or lasers in confocal or wide-field modes. Achieve quantitative, multiparameter cell imaging and analysis simultaneously with HCA.

Learn more

Varioskan LUX Multimode Microplate Reader

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including fluorescence, absorbance, luminescence, AlphaScreen tools, and time-resolved fluorescence.

Learn more


Attune NxT Flow Cytometer

The Attune NxT Flow Cytometer system enables multiparametric flow cytometry to both new and experienced researchers. Using acoustic assisted hydrodynamic focusing technology to accelerate experimental run times, this compact flow cytometer offers rapid and accurate analysis for a broad range of sample types including fluorescent-labeled cells.

  • Efficient—up to 10X faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors; up to 6 channels off the violet laser
  • Transformative—run difficult samples with a system that is less sensitive to clogging

Learn more

B-27 Plus Neuronal Culture System

B-27 Plus Neuronal Culture System is a next-generation media system designed to give highest rate of in vitro survival of primary rodent and human stem cell–derived neurons.

  • At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
  • Improved electrophysiological activity and maturation
  • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Learn more


FluoroBrite DMEM

Gibco FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health.

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Learn more


Fluorescent cell imaging reagents

Fluorescent cell imaging reagents
A 60 sec time course shows the resistance to photobleaching achieved with ProLong Glass antifade mountant. Fixed HeLa cells were labeled with Invitrogen Fluorescein Phalloidin and mounted in ProLong Glass antifade mountant or 50% phosphate-buffered saline (PBS)/glycerol solution. Images were acquired at 12 sec intervals using a 20x objective with continuous illumination from a standard 100 W Hg-arc lamp.

We offer innovative Invitrogen fluorescent reagents for cell and protein labeling that can help you get publication-quality images easily, the first time.

Reagents for 5-Steps to Publication-Quality Images of Fixed Cells
Reagents for Live-Cell Imaging


Dishes and plates for high-quality imaging

Thermo Scientific Nunc glass-bottom dishes—a polystyrene fixture combined with a glass-coverslip bottom offers the optimum optical characteristics required for high-magnification microscopy and confocal image analysis.

Thermo Scientific Nunc 96- and 384-well optical-bottom plates—cell culture–treated coverglass-based plates available with several surface-treatment options and are designed to deliver optimum clarity with minimum light scatter and autofluorescence background.

Thermo Scientific Nunc Lab-Tek Chamber Slide and Chambered Coverglass—seed, incubate, fix, and stain on a single microscope slide using the chamber slide system with removable chamber; ideal for high-magnification microscopy and confocal image analysis.


Opti-MEM Reduced Serum Medium

Gibco Opti-MEM I Reduced-Serum Medium (MEM) is an improved MEM that allows for a reduction of fetal bovine serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine reagents.

Learn more

Lipofectamine reagent family

Lipofectamine reagent family
Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours posttransfection. Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines.

Invitrogen Lipofectamine transfection reagents are designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures.


Neon Transfection System

Invitrogen Neon Transfection System is a simple, customizable, and gentle electroporation instrument designed for high transfection efficiency

  • Superior results—effective in difficult cells, including T and B cells
  • Customizable—preprogrammed 24-well optimization protocols and open platform for additional protocols
  • Simple—the pipette based system is easy to use allows for increased cell viability compared to traditional cuvette based systems

Learn more

Invitrogen antibodies

Count on Invitrogen antibodies, verified with our 2-part testing approach to help ensure consistent results and reproducibility. We offer more than 83,000 primary and secondary antibodies covering over 91% of the humane proteome. 

Search all antibodies

SuperBright primary antibodies

Invitrogen eBioscience Super Bright antibodies are a superior fluorophore choice for your flow cytometer’s violet laser. They have exceptional brightness and photostability, low background, and allow for greater utilization of the violet laser by enabling detection channels from 436 nm to 702 nm. For cells with low expressed target proteins, these bright reagents can provide the difference to detect and resolve dim populations.

Learn more


Alexa Fluor Plus secondary antibodies

Alexa Fluor Plus secondary antibodies were designed to help you detect and visualize low-abundance targets in rare or precious samples. The unique proprietary “plus” dye chemistry coupled with high pre-adsorption offers up to 4x higher signal-to-noise, minimizes cross-reactivity and yields increased sensitivity to provide more detail.

Learn more

Countess II FL Automated Cell Counter

A benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability quickly and easy.

Learn more


EVOS FL Auto 2 Imaging System

Supports high-resolution mosaic tiling, Z-stacking, multi-position well scanning, cell counting with thresholding, live cell imaging, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage, and high quality optics enable you to produce publication-quality images in seconds.

Learn more


CellInsight CX7 and CX7 LZR High-Content Analysis (HCA) Platforms

Thermo Scientific CellInsight CX7 and CX7 LZR HCA Platforms offer an integrated way to develop and automate high-content assays using a combination of bright-field and 7-color fluorescent imaging with LEDs or lasers in confocal or wide-field modes. Achieve quantitative, multiparameter cell imaging and analysis simultaneously with HCA.

Learn more

Varioskan LUX Multimode Microplate Reader

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including fluorescence, absorbance, luminescence, AlphaScreen tools, and time-resolved fluorescence.

Learn more


Attune NxT Flow Cytometer

The Attune NxT Flow Cytometer system enables multiparametric flow cytometry to both new and experienced researchers. Using acoustic assisted hydrodynamic focusing technology to accelerate experimental run times, this compact flow cytometer offers rapid and accurate analysis for a broad range of sample types including fluorescent-labeled cells.

  • Efficient—up to 10X faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors; up to 6 channels off the violet laser
  • Transformative—run difficult samples with a system that is less sensitive to clogging

Learn more

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** This promotion is open to life science professionals in the US (excluding Puerto Rico) and Canada who are 21 years or older. Sample requests must be received by the end date of each sampling program or until sample supplies are depleted. See full terms and conditions, name, and Cat. No. of the sample on the sample request website. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.