Probe Type Application Starting
Material for Probe Synthesis
Probe
Synthesis
Reaction
Ambion Kit Comments
dsDNA (I,N) Northern, Southern, dot blots cDNA inserts, PCR product, plasmid with insert Random priming reaction DECAprime™ II
  • Fastest reaction
  • Least sensitive
ssRNA (I,N) NPA, in situ hybridization Plasmid with insert and polymerase promoter In vitro transcription MAXIscript™
  • For use in ribonuclease protection assay
Oligonucleotide (I) Northern, Southern, dot blots Oligonucleotide End labeling KinaseMax™
  • Fastest reaction
  • Least sensitive
NPA Oligonucleotide End labeling KinaseMax™
  • Fastest reaction
  • Least sensitive
ssRNA
ssDNA
dsDNA
Oligonucleotide (N)
Northern, Southern, Dot blots, in situ hybridization DNA, RNA or oligonucleotide Chemical labeling, post-nucleic acid synthesis BrightStar™ Psoralen-Biotin
  • Sensitivity depends on nucleic acid labeled (e.g., ssRNA vs. dsDNA)



(I) Isotopic
(N) Nonisotopic