A number of different methods, technologies, and protocols are available for performing the actual cloning reaction. To choose the one that best meets your needs, you will need to analyze the current parameters of your experiment and plan ahead for any downstream procedures. This section discusses several highly efficient cloning technologies:

The table below can help you navigate your way through these technologies.

  Restriction Enzyme Cloning TA-Cloning TOPO™ (TA, blunt, directional) Gateway™ Cloning Technology GeneArt™ Seamless Cloning GeneArt™ Type IIs Assembly GeneArt™High-Order Genetic Assembly
Fragments Cloned Simultaneously 1 1 1 Up to 4 Up to 4 Up to 8 Up to 10
Max Fragment (s) Size Variable 1-3 kb <5 kb
(<10 kb for XL-TOPO®)
Variable Up to 10 kb, max total size of 40 kb
Up to 10 kb, max total size of 13-20 kb
Up to 100 kb, max total size of 110 kb
Gene Shuttling Between Vectors w/o PCR or Restriction Enzymes NO NO NO YES NO NO NO
Seamless (No Extra Sequences) NO NO NO NO YES YES YES
Use Your Own Vector YES NO NO YES (may require conversion)
Time to Clone Multiple Fragments Days to Weeks Not Possible Not
>4 Days 1 hr 1 hr 3 Days
4 Fragment Cloning Efficiency NA NA NA 30% - 85% 75% >90% >90%
Web-Based Vector Design Tool NO NO NO NO YES YES YES

Browse for vectors using our Vector selection tool