Useful Numbers for Cell Culture
Useful information for various sizes of cell culture dishes and flasks
There are various sizes of dishes and flasks used for cell culture. Some useful numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.
| Surface area (cm2) | Seeding density | Cells at confluency1 | Versene (mL of 0.05% EDTA) | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA) | Growth medium (mL) | |
|---|---|---|---|---|---|---|
| Dishes | ||||||
| 35mm | 9 | 0.3 x 106 | 1.2 x 106 | 1 | 1 | 2 |
| 60mm | 28.2 | 0.8 x 106 | 3.2 x 106 | 3 | 2 | 3 |
| 100mm | 78.5 | 2.2 x 106 | 8.8 x 106 | 5 | 3 | 10 |
| 150mm | 176.7 | 5.0 x 106 | 20.0 x 106 | 10 | 8 | 20 |
| Culture plates | ||||||
| 6-well | 9 | 0.3 x 106 | 1.2 x 106 | 2 | 2 | 3–5 |
| 12-well | 4 | 0.1 x 106 | 0.4 x 106 | 1 | 1 | 1–2 |
| 24-well | 2 | 0.05 x 106 | 0.2 x 106 | 0.5 | 0.5 | 0.5–1.0 |
| Flasks | ||||||
| T-25 | 25 | 0.7 x 106 | 2.8 x 106 | 3 | 3 | 3–5 |
| T-75 | 75 | 2.1 x 106 | 8.4 x 106 | 5 | 5 | 8–15 |
| T-160 | 162 | 4.6 x 106 | 18.4 x 106 | 10 | 10 | 15–30 |
1The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used.
