Growth Factor Supplementation for Specific Cells

Growth and attachment factors, natural and recombinant, are used in conjunction with other essential nutrients and hormones to maximize biological stimulation.  The optimal conditions and concentrations of growth factor for particular cell types must be empirically determined.  The following table provides a summary of conditions for growth and attachment factor supplementation reported to give maximal stimulation for various cell lines.  It can be used to establish initial concentrations prior to optimization.

Cell Line Cell Type Growth or Attachment Factor Suggested Conc. (ng/ml) Medium Serum Suppl.
Human mammary carcinomaEGF(a)100DMEM2% FBS
Human foreskin fibroblastEGF(a)2DMEM10% FBS
Primary hepatocyteEGF(a)10DMEM10% FBS
Rat hepatic eptheliumEGF(a)10Richter's MEM10% FBS
Human cervical carcinomaEGF(a)2.5 x104DMEM10% FBS
A431Epidermal carcinomaEGF(a)2.5 x 104DMEM10% FBS
Primary human fibroblastEGF(a)1x103MEM Earle's
10% FBS
MKN-7Human adenocarcinomaEGF(a)50DMEM8% FBS
BALB/c 3T3Mouse fibroblastEGF(a)50DMEM/F-12--
TM-4Mouse testes epitheliumEGF(a)3 DMEM/F-12--
NRK-49FRat fibroblastEGF(a)50DMEM/F-12--
Rat neuroblastomabFGF1F-12--
BHK-21Baby hamster kidneybFGF3DMEM/F-125% Calf