Table 3.1 Molecular Probes azide and alkyne derivatives.

Compound or Fluorophore Azide or Alkyne Cat. No. Application
Azide- or Alkyne-Modified Biomolecules for Metabolic or Enzymatic Labeling
EdU (5-ethynyl-2'-deoxyuridine) alkyne A10044
E10187
E10415
Detection of nascent DNA synthesis
EU (5-ethynyl uridine) alkyne E10345 Detection of nascent RNA synthesis
Click-iT AHA (L-azidohomoalanine) azide C10102 Detection of nascent protein synthesis or inhibition
Click-iT HPG (L-homopropargylglycine) alkyne C10186
Click-iT farnesyl alcohol, azide azide C10248 Identification of isoprenylated proteins
Click-iT geranylgeranyl alcohol, azide azide C10249
Click-iT fucose alkyne (tetraacetylfucose alkyne) alkyne C10264 Identification of fucosylated proteins
Click-iT LAA (linoleamide alkyne) alkyne C10447 Identification of lipid peroxidation–derived protein modifications
Click-iT palmitic acid, azide azide C10265 Identification of protein fatty acylation
Click-iT myristic acid, azide azide C10268
Click-iT GalNAz (tetraacetylated N-azidoacetylgalactosamine) azide C33365 Identification of O-linked glycoproteins
Click-iT ManNAz (tetraacetylated N-azidoacetylmannosamine) azide C33366 Identification of sialic acid–modified glycoproteins
Click-iT GlcNAz (tetraacetylated N-azidoacetylglucosamine) azide C33367 Identification of O-linked glycoproteins
Azide- or Alkyne-Modified Fluorophores and Haptens for Detection (Ex/Em) *
Alexa Fluor 488 (495/519) azide A10266 Fluorescent dye or hapten †
alkyne A10267
DIBO alkyne C10405 Fluorescent dye (or hapten †) for copper-free labeling and detection of azide-modified targets
Alexa Fluor 555 (555/565) azide A20012 Fluorescent dye
alkyne A20013
DIBO alkyne C10406 Fluorescent dye for copper-free labeling and detection of azide-modified targets
Alexa Fluor 594 (590/617) azide A10270 Fluorescent dye
alkyne A10275
DIBO alkyne C10407 Fluorescent dye for copper-free labeling and detection of azide-modified targets
Alexa Fluor 647 (650/668) azide A10277 Fluorescent dye
alkyne A10278
DIBO alkyne C10408 Fluorescent dye for copper-free labeling and detection of azide-modified targets
Biotin (NA)‡ azide B10184 Hapten
alkyne B10185
DIBO alkyne C10412 Hapten for copper-free labeling and detection of azide-modified targets
Oregon Green 488 (496/524) azide O10180 Fluorescent dye or hapten †
alkyne O10181
Tetramethylrhodamine (TAMRA) (555/580) azide T10182 Fluorescent dye or hapten †
alkyne T10183
DIBO alkyne C10410 Fluorescent dye for copper-free labeling and detection of azide-modified targets
* Absorption and fluorescence emission maxima, in nm. † As hapten, use with anti-dye antibody; see Anti-Dye and Anti-Hapten Antibodies—Section 7.4. ‡ Requires streptavidin for detection; see Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices—Section 7.6.